A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice
| Autor: | Hee Kyung Hong, Katherine J. Motyl, Carla B. Green, Jeremy J. Stubblefield, Joseph S. Takahashi, Anyonya R. Guntur, Sheila Bornstein, Li Tian, Phuong T. Le, Clifford J. Rosen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Physiology Transgene Clinical Biochemistry Phosphatase Adipose tissue Mice Transgenic Mitochondrion Oxidative Phosphorylation Article Fats 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Adenosine Triphosphate Animals Humans RNA Messenger Nuclear protein Transcription factor Messenger RNA Adipogenesis Chemistry Nuclear Proteins Cell Differentiation Mesenchymal Stem Cells Cell Biology Cell biology Mitochondria Mice Inbred C57BL PPAR gamma 030104 developmental biology HEK293 Cells Adipose Tissue 030220 oncology & carcinogenesis Models Animal Adenosine triphosphate Transcription Factors |
| Zdroj: | J Cell Physiol |
| ISSN: | 1097-4652 |
| Popis: | Nocturnin (NOCT) belongs to the Mg2+ dependent Exonucleases, Endonucleases, Phosphatase (EEP) family of enzymes that exhibit various functions in vitro and in vivo. NOCT is known to function as a deadenylase, cleaving poly-A tails from mRNA (messenger RNA) transcripts. Previously, we reported a role for NOCT in regulating bone marrow stromal cell differentiation through its interactions with PPARγ. In this study, we characterized the skeletal and adipose tissue phenotype when we globally overexpressed Noct in vivo. After 12 weeks of Noct overexpression, transgenic male mice had lower fat mass compared to controls, with no significant differences in the skeleton. Based on the presence of a mitochondrial target sequence in NOCT, we determined that mouse NOCT protein localizes to the mitochondria; subsequently, we found that NOCT overexpression led to a significant increase in the preadipocytes ability to utilize oxidative phosphorylation for ATP (adenosine triphosphate) generation. In summary, the effects of NOCT on adipocytes are likely through its novel role as a mediator of mitochondrial function. |
| Databáze: | OpenAIRE |
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