What makes thienoguanosine an outstanding fluorescent DNA probe?
| Autor: | Yitzhak Tor, Ludovic Richert, Yves Mély, Jagannath Kuchlyan, Stefano Ciaco, Pascal Didier, Lara Martínez-Fernández, Krishna Gavvala, Roberto Improta, Christian Boudier, Mattia Mori |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Guanosine
Quantum yield Bioengineering Molecular Dynamics Simulation 010402 general chemistry 01 natural sciences Biochemistry Fluorescence Catalysis Fluorescence spectroscopy Article Nucleobase Molecular dynamics chemistry.chemical_compound Structure-Activity Relationship Colloid and Surface Chemistry AP site Fluorescent Dyes Spectrometry Chemistry General Chemistry DNA 0104 chemical sciences Kinetics Spectrometry Fluorescence Chemical physics Chemical Sciences Solvents Nucleic Acid Conformation DNA Probes |
| Zdroj: | J Am Chem Soc Journal of the American Chemical Society, vol 142, iss 40 |
| Popis: | Thienoguanosine ((th)G) is an isomorphic guanosine (G) surrogate that almost perfectly mimics G in nucleic acids. To exploit its full potential and lay the foundation for future applications, twenty DNA duplexes, where the bases facing and neighboring (th)G were systematically varied, were thoroughly studied using fluorescence spectroscopy, molecular dynamics simulations and mixed quantum mechanical/molecular mechanics calculations, yielding a comprehensive understanding of its photophysics in DNA. In matched duplexes, (th)G’s hypochromism was larger for flanking G/C residues but its fluorescence quantum yield (QY) and lifetime values were almost independent of the flanking bases. This was attributed to high duplex stability, which maintains a steady orientation and distance between nucleobases, so that a similar charge transfer (CT) mechanism governs the photophysics of (th)G independently of its flanking nucleobases. (th)G can therefore replace any G residue in matched duplexes, while always maintaining similar photophysical features. In contrast, the local destabilization induced by a mismatch or an abasic site restores a strong dependence of (th)G’s QY and lifetime values on its environmental context, depending on the CT route efficiency and solvent exposure of (th)G. Due to this exquisite sensitivity, (th)G appears ideal for monitoring local structural changes and single nucleotide polymorphism. Moreover, (th)G’s dominant fluorescence lifetime in DNA is unusually long (9–29 ns), facilitating its selective measurement in complex media using a lifetime-based or a time-gated detection scheme. Taken together, our data highlights (th)G as an outstanding emissive substitute for G with good QY, long fluorescence lifetimes and exquisite sensitivity to local structural changes. |
| Databáze: | OpenAIRE |
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