Type-Specific Detection of Echovirus 30 Isolates Using Degenerate Reverse Transcriptase PCR Primers
| Autor: | David R. Kilpatrick, M. Steven Oberste, Mark A. Pallansch, Jacqueline Quay |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Echovirus viruses Molecular Sequence Data Echovirus Infections Biology medicine.disease_cause law.invention Capsid Species Specificity law Virology medicine Humans Amino Acid Sequence Peptide sequence Polymerase chain reaction DNA Primers Reverse Transcriptase Polymerase Chain Reaction Molecular biology Inosine Reverse transcriptase Enterovirus B Human Reverse transcription polymerase chain reaction Real-time polymerase chain reaction Enterovirus In silico PCR |
| Zdroj: | Journal of Clinical Microbiology. 39:1299-1302 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.39.4.1299-1302.2001 |
| Popis: | Following an approach used to specifically identify polioviruses and enterovirus 71, we have developed reverse transcriptase (RT) PCR primers containing mixed-base residues or deoxyinosine at positions of codon degeneracy. These primers permit specific RT-PCR amplification of echovirus 30 (E30) sequences by targeting sites that encode conserved amino acid motifs within the major capsid protein, VP1. All 221 E30 strains tested, isolated in 16 countries over a 44-year period, yielded the predicted 158-bp PCR product. No specific products were obtained by PCR assays containing templates from any of the other 63 EV serotypes. Inosine-containing degenerate primers may be widely applicable to the identification of echovirus serotypes by PCR. |
| Databáze: | OpenAIRE |
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