Antifungal and Synergistic Effects of the Ethyl Acetate Extract of Tanacetum vulgare (L.) Against Candida albicans
| Autor: | Ferit Koçani, Blerim Kamberi, Ariana Kameri, Arsim Kurti, Kemajl Kurteshi, Zeqir Hashani, Arben Haziri |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
Antifungal Agents Ethyl acetate Microbial Sensitivity Tests 030204 cardiovascular system & hematology Acetates Laboratory Research Endodontics 03 medical and health sciences chemistry.chemical_compound Tanacetum 0302 clinical medicine food Candida albicans medicine Agar Humans Agar diffusion test Lymphocytes Chromatography Calcium hydroxide biology Plant Extracts Chlorhexidine Drug Synergism General Medicine biology.organism_classification Corpus albicans chemistry 030220 oncology & carcinogenesis Sodium hypochlorite Dentin medicine.drug Mutagens |
| Zdroj: | Medical Science Monitor Basic Research |
| ISSN: | 2325-4416 |
| Popis: | BACKGROUND With the continued demand for new, effective, and safe endodontic therapies, the aim of this study was assessment of efficiency of the ethyl acetate (EthOAc) extract of Tanacetum vulgare (L.) against Candida albicans. MATERIAL AND METHODS The antifungal effectiveness of the EthOAc extract of T. vulgare was determined using the agar disk diffusion method. The inhibition zones induced by the EthOAc extract were compared after 5 minutes, 60 minutes, and 24 hours to those induced by standard solutions (2% chlorhexidine, saturated calcium hydroxide, and 2% sodium hypochlorite). Statistical analysis of the results was performed using the Kruskal-Wallis test and one-way ANOVA. RESULTS The inhibition zone of chlorhexidine against C. albicans was 30.3-19.3 mm, but in combination with EthOAc extract (100 mg/mL) of T. vulgare, this inhibition was from 32.7-30 mm, indicating that this combination exerted a marked synergistic effect against C. albicans. The inhibition zone of sodium hypochlorite (69.7-65 mm) was higher than the inhibition zone of EthOAc extract and chlorhexidine. The combination of EthOAc extract with sodium hypochlorite resulted in a loss of antifungal activity. Furthermore, the activity of the EthOAc extract against C. albicans was decreased after mixing the extract with dentine at a concentration of 25 mg/50 µL (30.3-20.7 mm). The EthOAc extract did not show a genotoxic effect on lymphocyte cells. CONCLUSIONS The EthOAc extract of T. vulgare may be a useful tool to discover natural bioactive agents that have antifungal activity against C. albicans and could be used as endodontic therapies. |
| Databáze: | OpenAIRE |
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