Insecticide susceptibility and detoxication enzyme activities among Coptotermes formosanus Shiraki workers sampled from different locations in New Orleans
Autor: | W. David Woodson, Steven M. Valles |
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Rok vydání: | 2002 |
Předmět: |
Insecticides
Physiology Health Toxicology and Mutagenesis Drug Resistance Context (language use) Chlordane Isoptera Toxicology Biochemistry Esterase Cypermethrin chemistry.chemical_compound Coptotermes Pyrethrins Animals Aldrin Glutathione Transferase biology Esterases Cell Biology General Medicine biology.organism_classification chemistry Chlordan Chlorpyrifos Inactivation Metabolic Biological Assay Formosan subterranean termite |
Zdroj: | Comparative biochemistry and physiology. Toxicologypharmacology : CBP. 131(4) |
ISSN: | 1532-0456 |
Popis: | Coptotermes formosanus Shiraki worker termites were sampled from 20 locations in the City Park area of New Orleans, LA. The termites were subsequently assayed to determine their susceptibility to cypermethrin, chlordane and chlorpyrifos, and detoxication enzyme activity. Cypermethrin was most toxic against Formosan subterranean termite workers, chlorpyrifos exhibited intermediate toxicity and chlordane was least toxic. A comparison of insecticide susceptibility between the most and least tolerant colonies revealed 1.9-, 1.7- and 1.8-fold differences in susceptibility for cypermethrin, chlorpyrifos and chlordane, respectively. As with the bioassay data, although significant differences were noted, a great deal of overlap was observed among the colonies for total cytochrome P450 content (difference of 2.2-fold between high and low value) aldrin epoxidation (3.6-fold) and cytosolic esterase (3.9-fold) activity. No significant differences were observed among the colonies for methoxyresorufin O-demethylase or glutathione S-transferase activity. Conversely, microsomal esterase activity varied greatly; a 38-fold difference was observed between the most (Cf1776) and least (Cf1387) active colonies. However, no significant correlation was observed between insecticide susceptibility and microsomal esterase activity. In fact, no significant correlations were observed between any of the enzyme activities measured and insecticide susceptibility. These results are discussed in the context of insecticide selection and future control effectiveness. |
Databáze: | OpenAIRE |
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