High-throughput sequencing-based analysis of the composition and diversity of endophytic bacterial community in seeds of upland rice
| Autor: | Zhishan Wang, Yongqiang Zhu, Xiaoxia Zhang, Yang Liu, Weiping Wang, Xianyu Wu, Ni Li, Ruixue Jing, Hai Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Root system
Upland rice Biochemistry Microbiology Endophyte Crop 03 medical and health sciences Soil Microbial ecology Genetics Endophytes Molecular Biology 030304 developmental biology 0303 health sciences biology Ecotype Bacteria 030306 microbiology food and beverages Sowing Genetic Variation High-Throughput Nucleotide Sequencing Oryza General Medicine biology.organism_classification Agronomy Seeds Proteobacteria |
| Zdroj: | Archives of microbiology. 203(2) |
| ISSN: | 1432-072X |
| Popis: | Upland rice is an ecotype crop formed by long-term domestication and evolution of rice in the dry land without water layer. Generally, its stem and leaf are thick and luxuriant, its leaf is wide and light, its root system is developed, its root hair is abundant, its osmotic pressure of root and cell juice concentration of leaf are high, and it is drought resistant, heat-resistant and water absorbing. The purpose of this study is to reveal the “core flora” of endophytes in upland rice seeds by studying the diversity and community structure of endophytes in upland rice seeds, and to reveal the impact of soil environment on the formation of endophyte community structure in upland rice seeds by comparing with soil environment microorganisms in upland rice habitats. In this study, the high-throughput sequencing technology based on the Illumina Hiseq 2500 platform was used to study the structure and diversity of endophytic bacterial communities using upland rice varieties collected in different places and soil samples from their unified planting sites as materials. There are 42 endophytic OTUs coexisted in the 14 samples. At the phylum level, the first dominant phyla was Proteobacteria (93.81–99.99%) in all 14 samples. At the genus level, Pantoea (8.77% -87.77%), Pseudomonas (1.15–61.58%), Methylobacterium (0.40–4.64%), Sphingomonas (0.26–3.85%), Microbacterium (0.01–4.67%) and Aurantimonas (0.04–4.34%), which are probably the core microflora in upland rice seeds, served as the dominant genera that coexisted in all upland rice seeds tested. Compared with the soil microbial community structure in the upland rice uniform planting site, it was found that it had little effect on the endophytic community structure in upland rice seeds. This study is of great significance for the isolation, screening, functional evaluation and re-action of some functional microorganisms in upland rice in order to improve its agronomic traits. It also provides a certain reference for the interaction between microorganisms and plants. |
| Databáze: | OpenAIRE |
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