Progranulin serum levels and gene expression in subcutaneous vs visceral adipose tissue of severely obese patients undergoing bariatric surgery
| Autor: | Annette Hauenschild, Jens Albrecht, Judith Brock, Barbara Schmidt, Miriam Thomalla, Secil Akinci, Jutta Schlegel, Andreas Schmid, Efthymia Arapogianni, Andreas O. Schultz, Petra Ina Pfefferle, Thomas Karrasch, Inga Busse, Maria Koukou, Johannes Kruse, Andreas Schäffler, Winfried Padberg |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Subcutaneous Fat Adipose tissue Adipokine Bariatric Surgery Gene Expression 030209 endocrinology & metabolism Inflammation Intra-Abdominal Fat 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin resistance Progranulins Paired samples Internal medicine mental disorders Gene expression medicine Humans Obesity RNA Messenger Caloric Restriction business.industry Middle Aged medicine.disease Surgery 030220 oncology & carcinogenesis Cohort Female medicine.symptom business |
| Zdroj: | Clinical endocrinologyREFERENCES. 91(3) |
| ISSN: | 1365-2265 |
| Popis: | BACKGROUND Progranulin represents an adipokine putatively mediating insulin resistance and inflammation. Data in humans are sparse, and the source of circulating progranulin in obesity is unknown. OBJECTIVES Serum progranulin concentrations and subcutaneous (sc) as well as visceral (vis) adipose tissue (AT) progranulin expression were quantified in a large cohort of patients with obesity undergoing bariatric surgery (BS) (n = 153) or a low-calorie diet (LCD) (n = 121). COHORTS AND METHODS Paired serum and AT mRNA samples were obtained from patients with severe obesity undergoing BS (ROBS cohort; Research in Obesity and Bariatric Surgery). Serum progranulin was measured by ELISA in both cohorts, and AT mRNA expression was analysed by quantitative real-time PCR in bariatric patients. RESULTS There was no gender-specific effect in serum progranulin or AT progranulin expression. Importantly, circulating progranulin was independent from adipose tissue gene expression in paired samples. sc AT progranulin expression was higher than in vis AT (P = 0.027), and there was a positive correlation between sc AT and vis AT gene expression (P |
| Databáze: | OpenAIRE |
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