Miniaturized multiparametric flexible platform for the simultaneous monitoring of ionic: Application in real urine

Autor: Gemma Gabriel, Ana Moya, Rosa Villa, Yoskaly Lazo-Fernandez, Abdelhamid Errachid, Ignacio Gimenez, Xavi Illa
Přispěvatelé: Instituto de Microelectrònica de Barcelona (IMB-CNM), Centro Nacional de Microelectronica [Spain] (CNM)-Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Instituto Aragonés de Ciencias de la Salud [Zaragoza] (IACS), Micro & Nanobiotechnologies, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), This work was partially done with the support of the Spanish government funded projects DPI2011-28262-C04 and DPI2015-65401-C3-3-R (MINECO/FEDER, EU) and BFU2007-62119 (MINECO). Also, the authors acknowledge the financial support from the European Union’s Horizon 2020 research and innovation programe entitled MicroMole and HEARTEN grant agreement No 653626 and No 643694 respectively. The authors also acknowledge ICTS 'NANBIOSIS', more specifically by the SU8 Unit of the CIBER in Bioengineering, Biomaterials & Nanomedicne (CIBER-BBN) at the IMB-CNM (CSIC). This work has also made use of the Spanish ICTS Network MICRONANOFABS partially supported by MEINCOM., European Project: 653626,H2020,H2020-FCT-2014,microMole(2015), European Project: 643694,H2020,H2020-PHC-2014-single-stage,HEARTEN(2015), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2018
Předmět:
Zdroj: Sensors and Actuators B: Chemical
Sensors and Actuators B: Chemical, Elsevier, 2018, 255, pp.2861-2870. ⟨10.1016/j.snb.2017.09.104⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Sensors and Actuators B: Chemical, 2018, 255, pp.2861-2870. ⟨10.1016/j.snb.2017.09.104⟩
Zaguán. Repositorio Digital de la Universidad de Zaragoza
ISSN: 0925-4005
DOI: 10.1016/j.snb.2017.09.104
Popis: Biomonitoring is a research topic that has largely relied on cell culture systems. Recently, the development of “Organ-on-a-Chip” (OC) platforms and the need for a continuous monitoring of these systems has increased its interest. However, the biomonitorization in these systems is still at its infancy due to the difficulty to adapt the sensors to microfluidic OC systems. In this work we have fabricated a modular, versatile, scalable, multi-analyte sensing platform, which integrates dissolved oxygen (DO), Na, K and pH sensors for monitoring their concentrations in small volume of biological fluids. The platform is fabricated by means of rapid prototyping techniques using polymeric substrates, and incorporates all the necessary elements to measure the four analytes. It was designed with four gold sensing electrodes, and integrates a gold counter electrode and an Ag/AgCl pseudo-reference electrode for the electrochemical measurements in small volumes (0.83 μL) of samples. The sensors featured good sensitivities of 3.60 ± 0.2 nA (mg L) for DO and 69 ± 1 mV decade for pH. Na and K μISE exhibited sensitivities of 57 ± 1 mV decade and 52 ± 2 mV decade, and low limits of detection 5 × 10 M and 0.5 × 10 M respectively. This platform allows the dynamic measurement of the biological fluids parameters simultaneously, in real time and with a rapid response. The versatility of the platform allows its adaptation in any microfluidic cells culture systems. The novel multi-sensor platform has been validated in controlled buffers and with artificial urine (AU). A proof-of-concept using real mice urine (RU) has been carried out, demonstrating the good behaviour of the multi-sensing platform.
Databáze: OpenAIRE
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