Detection of Mycobacterium leprae in saliva and the evaluation of oral sensitivity in patients with leprosy
| Autor: | Felicien Gonçalves Vasquez, Fernanda Borowsky da Rosa, Tatiana Amaral Pires de Almeida, Valdinete Alves do Nascimento, Felipe Gomes Naveca, Maria da Graça Souza Cunha, Victor Costa de Souza |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Adult DNA Bacterial Male Saliva lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 real-time polymerase chain reaction lcsh:QR1-502 Biology Oral cavity lcsh:Microbiology sensation disorders Leprosy medicine Humans In patient Mycobacterium leprae Articles Middle Aged biology.organism_classification medicine.disease stomatognathic diseases Real-time polymerase chain reaction Cross-Sectional Studies Case-Control Studies Immunology oral cavity Female Sample collection leprosy |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 5, Pp 572-577 (2013) Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 5, Pages: 572-577, Published: AUG 2013 |
| ISSN: | 1678-8060 0074-0276 |
| Popis: | The aim of this study was to investigate sensitivity disorders in the oral cavity related to the presence of Mycobacterium leprae in the saliva of treatment-naïve patients with leprosy in the state of Amazonas, Brazil. A cross-sectional study was conducted involving 45 subjects with leprosy. The subjects were interviewed to evaluate the sensitivity of the oral cavity. For the detection of M. leprae, saliva and slit-skin smear samples were collected. The samples were analysed using a bacteriological index (BI) protocol and the real-time quantitative polymerase chain reaction (qPCR). The results indicated that 15 of the 45 (33.3%) subjects with leprosy showed decreased oral sensitivity, which confirmed the importance of the oral cavity sensitivity evaluation. There was not a direct relationship between the presence of M. leprae in saliva and changes in oral sensitivity. Positive saliva qPCR results from six (31.6%) of 19 paucibacillary (PB) patients suggested the possibility of a new site for sample collection. Positive results using these diagnostic techniques (BI, slit-skin smear and saliva qPCR) increased to 55.5%, thus opening the possibility of combining these different techniques to increase the rate of positive diagnoses, especially in PB patients. |
| Databáze: | OpenAIRE |
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