Sohlh1 is required for synaptonemal complex formation by transcriptionally regulating meiotic genes during spermatogenesis in mice
| Autor: | Wanwei Dong, Wanjing Qi, Jia Liu, Meiyu Jin, Xue Zhang, Gongqing Liu, Qi Sun, Yuan Li, Zhihong Zheng, Bing Du |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Transcriptional Activation Cell Cycle Proteins Biology 03 medical and health sciences Mice 0302 clinical medicine Meiosis Spermatocytes Genetics Transcriptional regulation Basic Helix-Loop-Helix Transcription Factors Animals Spermatogenesis Gene Transcription factor Mice Knockout 030219 obstetrics & reproductive medicine Synaptonemal Complex Zygotene Stage Synapsis Cell Biology Cell biology DNA-Binding Proteins Synaptonemal complex 030104 developmental biology Developmental Biology |
| Zdroj: | Molecular reproduction and development. 86(3) |
| ISSN: | 1098-2795 |
| Popis: | Gonad-specific transcription factor spermatogenesis- and oogenesis-specific helix-loop-helix transcription factor 1 (SOHLH1) plays a key role in the transcriptional regulation of the expression of differentiating spermatogonial genes. However, its role in spermatocytes (meiotic male germ cells) remains largely unknown. In this study, Sohlh1 knockout (KO) male mice displayed meiotic defects at the zygotene stage during spermatogenesis. Microarray analyses identified 66 upregulated genes and 139 downregulated genes in Sohlh1 KO testes compared with those in wild-type testes at postnatal Day 7.5. Among many of the downregulated genes, Sycp1 and Sycp3, which encode synaptonemal complex proteins 1 and 3 (SYCP1 and SYCP3), respectively, were significantly reduced in Sohlh1 knockout mice. Transmission electron microscopy revealed no formation of the synaptonemal complex in Sohlh1 KO spermatocytes. Luciferase reporter and chromatin-immunoprecipitation assays demonstrated that SOHLH1 enhanced the expression of the Sycp1 and Sycp3 genes by binding the -1276, -708, and -94 basepairs (bp) E-boxes upstream of the Sycp1 promoter and the -64 and -43 bp E-boxes upstream of the Sycp3 promoter. Our data suggest that SOHLH1 transcriptionally regulates the expression of many target genes critical for the meiotic phase of spermatogenesis. |
| Databáze: | OpenAIRE |
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