Localization of histamine H4 receptors in the central nervous system of human and rat
| Autor: | Robert S. Bitner, Arlene M. Manelli, Marina I. Strakhova, Jorge D. Brioni, Gin C. Hsieh, Timothy A. Esbenshade, Arthur L. Nikkel |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Cerebellum Pathology medicine.medical_specialty Central nervous system Hippocampus Biology Receptors G-Protein-Coupled Rats Sprague-Dawley Histamine receptor Ganglia Spinal medicine Animals Humans RNA Messenger Histamine H4 receptor Molecular Biology Cells Cultured Receptors Histamine H4 Neurons Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Brain Spinal cord Immunohistochemistry Rats Lumbar Spinal Cord medicine.anatomical_structure Liver Spinal Cord nervous system Receptors Histamine Neurology (clinical) Brainstem Neuroglia Neuroscience Spleen Developmental Biology |
| Zdroj: | Brain Research. 1250:41-48 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2008.11.018 |
| Popis: | Existing data on the expression of H(4) histamine receptor in the CNS are conflicting and inconclusive. In this report, we present the results of experiments that were conducted in order to elucidate H(4) receptor expression and localization in the brain, spinal cord, and dorsal root ganglia (DRG). Here we show that transcripts of H(4) receptor are present in all analyzed regions of the human CNS, including spinal cord, hippocampus, cortex, thalamus and amygdala, with the highest levels of H(4) mRNA detected in the spinal cord. In rat, H(4) mRNA was detected in cortex, cerebellum, brainstem, amygdala, thalamus and striatum. Very low levels of H(4) mRNA were detected in hypothalamus, and no H(4) signal was detected in the rat hippocampus. Fairly low levels of H(4) mRNA were detected in examined peripheral tissues including spleen and liver. Interestingly, strong expression of H(4) mRNA was detected in the rat DRG and spinal cord. Immunohistochemical analysis revealed expression of H(4) receptors on neurons in the rat lumbar DRG and in the lumbar spinal cord. Our observations provide evidence of the H(4) presence in both human and rodent CNS and offer some insight into possible role of H(4) in itch and pain. |
| Databáze: | OpenAIRE |
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