Distinct Roles of the ATR Kinase and the Mre11-Rad50-Nbs1 Complex in the Maintenance of Chromosomal Stability in Arabidopsis
| Autor: | Elisabeth Allain, Charles I. White, Maria Eugenia Gallego, Cyril Charbonnel, Simon Amiard, Annie Depeiges |
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| Přispěvatelé: | Laboratoire de Biologie Moléculaire de la Cellule (LBMC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), White, Charles, École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL) |
| Rok vydání: | 2010 |
| Předmět: |
0106 biological sciences
Arabidopsis [SDV.GEN] Life Sciences [q-bio]/Genetics Plant Science 01 natural sciences Histones chemistry.chemical_compound MESH: Arabidopsis Phosphorylation Research Articles MESH: Histones MRE11 Homologue Protein 0303 health sciences biology Cell cycle Protein-Serine-Threonine Kinases DNA-Binding Proteins Histone biological phenomena cell phenomena and immunity DNA damage MESH: Arabidopsis Proteins Protein Serine-Threonine Kinases MESH: Protein-Serine-Threonine Kinases MESH: Chromosomal Instability 03 medical and health sciences Chromosomal Instability Insertional Mitosis 030304 developmental biology MESH: DNA Damage [SDV.GEN]Life Sciences [q-bio]/Genetics MESH: Phosphorylation Arabidopsis Proteins Cell Biology MESH: Gamma Rays biology.organism_classification Molecular biology Mutagenesis Insertional enzymes and coenzymes (carbohydrates) MESH: Mutagenesis Insertional MRN complex chemistry Mutagenesis Gamma Rays Rad50 biology.protein MESH: DNA-Binding Proteins DNA DNA Damage 010606 plant biology & botany |
| Zdroj: | The Plant cell The Plant cell, 2010, 22 (9), pp.3020-33. ⟨10.1105/tpc.110.078527⟩ The Plant cell, American Society of Plant Biologists (ASPB), 2010, 22 (9), pp.3020-33. ⟨10.1105/tpc.110.078527⟩ |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.110.078527 |
| Popis: | International audience; Signaling of chromosomal DNA breaks is of primary importance for initiation of repair and, thus, for global genomic stability. Although the Mre11-Rad50-Nbs1 (MRN) complex is the first sensor of double-strand breaks, its role in double-strand break (DSB) signaling is not fully understood. We report the absence of γ-ray-induced, ATM/ATR-dependent histone H2AX phosphorylation in Arabidopsis thaliana rad50 and mre11 mutants, confirming that the MRN complex is required for H2AX phosphorylation by the ATM and ATR kinases in response to irradiation-induced DSB in Arabidopsis. rad50 and mre11 mutants spontaneously activate a DNA damage response, as shown by the presence of γ-H2AX foci and activation of cell cycle arrest in nonirradiated plants. This response is ATR dependent as shown both by the absence of these spontaneous foci and by the wild-type mitotic indices of double rad50 atr and mre11 atr plants. EdU S-phase labeling and fluorescence in situ hybridization analysis using specific subtelomeric probes point to a replicative S-phase origin of this chromosome damage in the double mutants and not to telomere destabilization. Thus, the data presented here show the exclusive involvement of ATR in DNA damage signaling in MRN mutants and provide evidence for a role for ATR in the avoidance of S-phase DNA damage. |
| Databáze: | OpenAIRE |
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