Non-destructive electron microscopy imaging and analysis of biological samples with graphene coating
| Autor: | Sangmin Kang, Konstantin S. Novoselov, Jong Bo Park, Byung Hee Hong, Seong-Min Kim, Irina Barbolina, Sung Pyo Cho, Je Min Yoo, Myung-Han Yoon, Yong-Jin Kim, Youngsoo Kim, Sang Jin Kim, Roman V. Gorbachev |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Materials science Scanning electron microscope Energy-dispersive X-ray spectroscopy Nanotechnology 02 engineering and technology Energy dispersive spectroscopy Graphene coating law.invention 03 medical and health sciences National Graphene Institute law Microscopy General Materials Science Sample preparation Biological sample Conductive Image resolution Graphene Mechanical Engineering General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 030104 developmental biology Mechanics of Materials Transmission electron microscopy ResearchInstitutes_Networks_Beacons/national_graphene_institute Electron microscope 0210 nano-technology Scanning electron microscopy |
| Zdroj: | Park, J B, Kim, Y J, Kim, S M, Yoo, J M, Kim, Y, Gorbachev, R, Barbolina, I I, Kim, S J, Kang, S, Yoon, M H, Cho, S P, Novoselov, K S & Hong, B H 2016, ' Non-destructive electron microscopy imaging and analysis of biological samples with graphene coating ', 2 D Materials, vol. 3, no. 4, 045004 . https://doi.org/10.1088/2053-1583/3/4/045004 2D Materials ResearcherID |
| ISSN: | 2053-1583 |
| DOI: | 10.1088/2053-1583/3/4/045004 |
| Popis: | In electron microscopy (EM), charging of non-conductive biological samples by focused electron beams hinders their high-resolution imaging. Gold or platinum coatings have been commonly used to prevent such sample charging, but it disables further quantitative and qualitative chemical analyses such as energy dispersive spectroscopy (EDS). Here we report that graphene-coating on biological samples enables non-destructive high-resolution imaging by EM as well as chemical analysis by EDS, utilizing graphene's transparency to electron beams, high conductivity, outstanding mechanical strength and flexibility. We believe that the graphene-coated imaging and analysis would provide us a new opportunity to explore various biological phenomena unseen before due to the limitation in sample preparation and image resolution, which will broaden our understanding on the life mechanism of various living organisms. |
| Databáze: | OpenAIRE |
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