Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures
| Autor: | Saara Korpela, Mikhail Vainshtein, Timo Korpela, Tatiana Abashina |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Microbiological Techniques Sucrose food.ingredient Density gradient ta3111 medicine.disease_cause 01 natural sciences Microbiology Gelatin 03 medical and health sciences chemistry.chemical_compound food medicine Centrifugation Density Gradient Escherichia coli Centrifugation Microwaves Molecular Biology Differential centrifugation Chromatography Chemistry Sucrose gradient 030104 developmental biology Microwave 010606 plant biology & botany |
| Zdroj: | Journal of microbiological methods. 153 |
| ISSN: | 1872-8359 |
| Popis: | Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM B-125 cells in discontinuous sucrose gradient which was optimized to obtain small round "nano-cells" free from other cell components. Gelatin added to the sucrose solutions had the advantages: i) accurate gradient layers were formed, ii) gelatin did not interfere with the cells and sub-particles during centrifugation, iii) solidified gelatin allowed to cut out the separated cell components, and iv) gelatin allowed to prepare ready-made solidified gradient centrifugation tubes into storage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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