| Autor: |
Ogbe, Ane, Kronsteiner, Barbara, Skelly, Donal T, Pace, Matthew, Brown, Anthony, Adland, Emily, Adair, Kareena, Akhter, Hossain Delowar, Ali, Mohammad, Ali, Serat-E, Angyal, Adrienn, Ansari, M Azim, Arancibia-Carcamo, Carolina V, Brown, Helen, Chinnakannan, Senthil, Conlon, Christopher, de Lara, Catherine, de Silva, Thushan, Dold, Christina, Dong, Tao, Donnison, Timothy, Eyre, David, Flaxman, Amy, Fletcher, Helen, Gardner, Joshua, Grist, James T, Hackstein, Carl-Philipp, Jaruthamsophon, Kanoot, Jeffery, Katie, Lambe, Teresa, Lee, Lian, Li, Wenqin, Lim, Nicholas, Matthews, Philippa C, Mentzer, Alexander J, Moore, Shona C, Naisbitt, Dean J, Ogese, Monday, Ogg, Graham, Openshaw, Peter, Pirmohamed, Munir, Pollard, Andrew J, Ramamurthy, Narayan, Rongkard, Patpong, Rowland-Jones, Sarah, Sampson, Oliver, Screaton, Gavin, Sette, Alessandro, Stafford, Lizzie, Thompson, Craig, Thomson, Paul J, Thwaites, Ryan, Vieira, Vinicius, Weiskopf, Daniela, Zacharopoulou, Panagiota, Chalk, Jeremy, Kerr, Georgina, Phalora, Prabhjeet, Csala, Anna, Jones, Mathew, Robinson, Nicola, Brown, Rachael, Hutchings, Claire, Provine, Nicholas, Ratcliff, Jeremy, Amini, Ali, Borak, Martyna, Dimitriadis, Stavros, Fordwoh, Thomas, Horsington, Bryn, Johnson, Sile, Morrow, Jordan, Warren, Yolanda, Wells, Charlie, Turtle, Lance, Klenerman, Paul, Goulder, Philip, Frater, John, Barnes, Eleanor, Dunachie, Susanna, Immunology, Oxford, Oxford, Protective TC |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Nature Communications
NATURE COMMUNICATIONS
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021) |
| ISSN: |
2041-1723 |
| Popis: |
Identification of protective T cell responses against SARS-CoV-2 requires distinguishing people infected with SARS-CoV-2 from those with cross-reactive immunity to other coronaviruses. Here we show a range of T cell assays that differentially capture immune function to characterise SARS-CoV-2 responses. Strong ex vivo ELISpot and proliferation responses to multiple antigens (including M, NP and ORF3) are found in 168 PCR-confirmed SARS-CoV-2 infected volunteers, but are rare in 119 uninfected volunteers. Highly exposed seronegative healthcare workers with recent COVID-19-compatible illness show T cell response patterns characteristic of infection. By contrast, >90% of convalescent or unexposed people show proliferation and cellular lactate responses to spike subunits S1/S2, indicating pre-existing cross-reactive T cell populations. The detection of T cell responses to SARS-CoV-2 is therefore critically dependent on assay and antigen selection. Memory responses to specific non-spike proteins provide a method to distinguish recent infection from pre-existing immunity in exposed populations.
Understanding the immune response to SARS-CoV-2 is dependent on being able to distinguish COVID-19 immune responses from cross-reactive immune responses to other coronaviruses. Here the authors show that choice of antigens and whether an ICS, ELISPOT or T cell proliferation assay is used has a major effect on this discriminatory ability. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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