Molecular Detection of Murine Norovirus from Experimentally and Spontaneously Infected Mice
Autor: | Shuko Kameda, Nobuhito Hayashimoto, Masahiko Yasuda, Toshio Itoh, Kazuo Goto, Akira Takakura, Tomoko Ishida |
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Rok vydání: | 2009 |
Předmět: |
Male
animal diseases ved/biology.organism_classification_rank.species Spleen Mice SCID Biology General Biochemistry Genetics and Molecular Biology Virus Rodent Diseases Feces Mice Cecum Fetus Mice Inbred NOD Pregnancy medicine Animals Caliciviridae Infections Mice Inbred ICR General Veterinary Reverse Transcriptase Polymerase Chain Reaction ved/biology Norovirus DNA-Directed RNA Polymerases Sequence Analysis DNA General Medicine Virology Reverse transcriptase Specific Pathogen-Free Organisms Reverse transcription polymerase chain reaction Disease Models Animal Fetal Diseases Real-time polymerase chain reaction medicine.anatomical_structure Molecular Diagnostic Techniques RNA Viral Female Animal Science and Zoology Murine norovirus |
Zdroj: | Experimental Animals. 58:135-140 |
ISSN: | 1881-7122 1341-1357 |
Popis: | The RNA polymerase gene of murine norovirus (MNV) was isolated from feces and organ samples by the reverse transcription (RT)-polymerase chain reaction (PCR). For experimental infection, homogenate of cecum obtained from an MNV-infected mouse was gavaged to 3 C.B-17-Prkdc(scid) (scid) mice and 3 ICR mice at 6 weeks of age. Sixty days after oral inoculation, MNV was isolated from the cecum (3/3 scid and 3/3 ICR), feces (3/3 scid and 3/3 ICR), duodenum (1/3 scid and 3/3 ICR), liver (1/3 scid and 1/3 ICR), and spleen (3/3 ICR) samples, but MNV was not detected in the brain, heart, kidney, lung, salivary gland, ovary, thymus, or uterus samples of any of the orally inoculated mice. Feces of males cohabiting with MNV infected dams were positive for viral RNA after 18 days of cohabitation, but 8 fetuses (embryonic day 18.0) derived from the dams were negative for the virus. The results suggest that the cecum and feces are the most suitable sample types for the detection of MNV in infected animals and that caesarean section is efficient for the elimination of the virus. In terms of spontaneous infection, the RNA polymerase gene of MNV was isolated from 33/245 (13.1%) cecum samples derived from 15/59 (25.4%) facilities, and the sequence analysis revealed that at least 5 types of the virus were prevalent. This is the first report on MNV infection in mouse colonies in Japan. |
Databáze: | OpenAIRE |
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