Protective effect of two Mycoplasma gallisepticum protein fractions affinity purified with monoclonal antibodies
| Autor: | Laszlo Stipkovits, U. Hellman, G. Czifra, Bo Sundquist |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Gel electrophoresis
Mycoplasma gallisepticum General Immunology and Microbiology Molecular mass biology Hemagglutination medicine.drug_class Sodium chemistry.chemical_element Monoclonal antibody biology.organism_classification Molecular biology Serum antibody Food Animals chemistry medicine Animal Science and Zoology Peptide sequence |
| Zdroj: | Avian pathology : journal of the W.V.P.A. 29(4) |
| ISSN: | 1465-3338 |
| Popis: | Two protein fractions of Mycoplasma gallisepticum (Mg) were affinity purified with monoclonal antibodies A3 and B3, and tested for protective capacity in chickens. One fraction, designated MgP1, appeared as a doublet of 64 and 62kDa bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis gels, while MgP2 consisted of five polypeptides (64, 56, 47, 45 and 43 kDa). The molecular mass, haemagglutination activity and matching amino acid sequence of MgP1 suggest that it is identical to pMGA1.2, the putative haemagglutinin of Mg. Groups of Mg-free chickens were immunized once or twice with 1 or 5 μ g MgP1 or MgP2, or a combination of the two, and adjuvanted with immunostimulating complexes. Except for the group given 1 μ g MgP1, all vaccinated groups showed a significant (P < 0.01) reduction in air sac lesions after challenge compared with unvaccinated controls. MgP2 appeared more protective than MgP1. Vaccination twice with MgP2 was the only regime that produced a detectable serum antibody response. |
| Databáze: | OpenAIRE |
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