SUMOylation Inhibition Mediated by Disruption of SUMO E1-E2 Interactions Confers Plant Susceptibility to Necrotrophic Fungal Pathogens
| Autor: | L. Maria Lois, Silvia Manrique, Arnaldo L. Schapire, Gaelle La Verde, Inês Teixeira, Natalia Rodrigo, María Coca, Laura Castaño-Miquel, Anna Perearnau, Abraham Mas, Bhagyasree N. Thampi, Josep Seguí |
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| Přispěvatelé: | European Research Council, Ministerio de Ciencia e Innovación (España), CSIC-IRTA-UAB-UB - Centre de Recerca Agrigenómica (CRAG), Ministerio de Educación, Cultura y Deporte (España), Generalitat de Catalunya |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient SUMO inhibition Protein domain SUMO protein Arabidopsis Plant Science Genetically modified crops Development environment and public health Flowering 03 medical and health sciences food Ascomycota Protein Domains Gene Expression Regulation Plant Botany Molecular Biology Botrytis cinerea Botrytis Plant Diseases chemistry.chemical_classification biology Arabidopsis Proteins Necrotrophic fungi food and beverages Ubiquitin-Protein Ligase Complexes E1-E2 interaction disruption biology.organism_classification Plants Genetically Modified Cell biology E1–E2 interaction disruption 030104 developmental biology Enzyme chemistry Mutagenesis Small Ubiquitin-Related Modifier Proteins E1-activating enzyme |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Digital.CSIC. Repositorio Institucional del CSIC |
| ISSN: | 1752-9867 |
| Popis: | Protein modification by SUMO modulates essential biological processes in eukaryotes. SUMOylation is facilitated by sequential action of the E1-activating, E2-conjugating, and E3-ligase enzymes. In plants, SUMO regulates plant development and stress responses, which are key determinants in agricultural productivity. To generate additional tools for advancing our knowledge about the SUMO biology, we have developed a strategy for inhibiting in vivo SUMO conjugation based on disruption of SUMO E1-E2 interactions through expression of E1 SAE2UFDCt domain. Targeted mutagenesis and phylogenetic analyses revealed that this inhibition involves a short motif in SAE2UFDCt highly divergent across kingdoms. Transgenic plants expressing the SAE2UFDCt domain displayed dose-dependent inhibition of SUMO conjugation, and have revealed the existence of a post-transcriptional mechanism that regulates SUMO E2 conjugating enzyme levels. Interestingly, these transgenic plants displayed increased susceptibility to necrotrophic fungal infections by Botrytis cinerea and Plectosphaerella cucumerina. Early after fungal inoculation, host SUMO conjugation was post-transcriptionally downregulated, suggesting that targeting SUMOylation machinery could constitute a novel mechanism for fungal pathogenicity. These findings support the role of SUMOylation as a mechanism involved in plant protection from environmental stresses. In addition, the strategy for inhibiting SUMO conjugation in vivo described in this study might be applicable in important crop plants and other non-plant organisms regardless of their genetic complexity. This work was supported by the European Research Council (ERC-2007-StG-205927) and the Spanish Ministry of Science (BIO2008-01495). L.C.-M., I.T., A.P., S.M., and N.R. were supported by research contracts through the CRAG. A.M. and J.S. were supported by predoctoral fellowships, Spanish Ministry of Education, Culture and Sport (FPU12/05292) and Ministry of Education and Science (BES-2005-6843), respectively, and A.L.S. was supported by Beatriu de Pinós post-doctoral grant of the Generalitat de Catalunya (2013 BP_B 00182). We also thank the Generalitat de Catalunya (Xarxa de Referència en Biotecnologia and 2009SGR 09626) for substantial support. |
| Databáze: | OpenAIRE |
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