Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants
| Autor: | Ditlev E. Brodersen, Jannie Assenholt, Torben Heick Jensen, John Mouaikel, Domenico Libri, Kasper R. Andersen |
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| Rok vydání: | 2008 |
| Předmět: |
Exonuclease
Saccharomyces cerevisiae Proteins Exosome complex THO complex RNA-binding protein Saccharomyces cerevisiae Fungal Proteins RNA Messenger RNA Processing Post-Transcriptional Nuclear protein Molecular Biology Sequence Deletion Ribonucleoprotein Messenger RNA Exosome Multienzyme Ribonuclease Complex biology Nuclear Proteins RNA-Binding Proteins RNA Fungal Molecular biology Protein Structure Tertiary Cell biology DNA-Binding Proteins Ribonucleoproteins Exoribonucleases biology.protein Reports |
| Zdroj: | Assenholt, J, Mouaikel, J, Andersen, K R, Brodersen, D E, Libri, D & Jensen, T H 2008, ' Exonucleolysis is required for nuclear mRNA quality control in yeast THO mutants ', RNA, vol. 14, no. 11, pp. 2305-13 . https://doi.org/10.1261/rna.1108008 |
| ISSN: | 1469-9001 1355-8382 |
| DOI: | 10.1261/rna.1108008 |
| Popis: | Production of aberrant messenger ribonucleoprotein particles (mRNPs) is subject to quality control (QC). In yeast strains carrying mutations of the THO complex, transcription induction triggers a number of interconnected QC phenotypes: (1) rapid degradation of several mRNAs; (2) retention of a fraction of THO-dependent mRNAs in transcription site-associated foci; and (3) formation of a high molecular weight DNA/protein complex in the 3′-ends of THO target genes. Here, we demonstrate that the 3′–5′ exonucleolytic domain of the nuclear exosome factor Rrp6p is necessary for establishing all QC phenotypes associated with THO mutations. The N terminus of Rrp6p is also important presumably through its binding to the Rrp6p co-factor Rrp47p. Interestingly, the 3′–5′ exonucleolytic activity of Dis3p, the only other active exonuclease of the nuclear exosome, can also contribute to RNA QC in THO mutants, while other nuclear 3′–5′ exonucleases cannot. Our data show that exonucleolytic attack by the nuclear exosome is needed both for provoking mRNP QC and for its ensuing elimination of faulty RNA. |
| Databáze: | OpenAIRE |
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