Effect of high temperature on the expressions of genes encoding starch synthesis enzymes in developing rice endosperms
| Autor: | Chunhai Shi, Zhao-wei Li, Zhenzhen Cao, Gang Pan, Ke-Su Wei, Fubiao Wang, Fangmin Cheng, Wei Geng |
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| Rok vydání: | 2015 |
| Předmět: |
Starch
Agriculture (General) Mutant Plant Science Gene mutation Biochemistry S1-972 high temperature chemistry.chemical_compound Food Animals Amylose Gene expression starch synthesis Gene Genetics Ecology biology rice Wild type food and beverages amylopectin strucrute chemistry gene expression biology.protein Animal Science and Zoology Starch synthase Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 14, Iss 4, Pp 642-659 (2015) |
| ISSN: | 2095-3119 |
| DOI: | 10.1016/s2095-3119(14)60782-6 |
| Popis: | High temperature is the major environmental factor affecting grain starch properties of cooking rice cultivars. In this study, two non-waxy indica rice genotypes, cv . 9311 and its mutant with extremely high amylose phenotype (9311 eha ) were used to study the differential expressions of genes in starch synthesis and their responses to high temperature (HT). Significant increase in apparent amylose content and hot-water-soluble starch content in mutant 9311 eha were genetically caused by a substitution from AGTTATA to AGGTATA at the leader intron 5′ splice site in Wx gene. This mutation resulted in different mRNA transcript levels, mRNA splicing efficiencies and protein levels of Wx between the two rice genotypes, which also lead to the genotype-dependent alteration in the temporal pattern of Wx transcription and granule-bound starch synthase (GBSS) activity in response to HT. However, changes in the activities of other starch synthesizing enzymes and their expressions of distinct isoform genes were not significant with the Wx gene mutation, thus only minor difference in the particle size of starch granule, chain-length distribution and gelatinization enthalpy were found between the two genotypes. The temporal-specific expression of multiple isoform genes responsive to different temperature regiments indicated that the reduction of GBSS transcript expression under HT was generally accompanied by the decreased expressions of SSSIIa, SSSIIIa and SBEIIb. Consequently, high temperature-ripened grains in 9311 eha showed high proportion of intermediate and long B chains and somewhat lower level of short A chain compared to the wildtype. The temperature-dependent alteration of amylose content was not only attributed to the reduced expression of GBSS, but also associated with the complimentary effect of SSSIIa and SBEIIb. |
| Databáze: | OpenAIRE |
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