Lycium barbarum extracts protect the brain from blood-brain barrier disruption and cerebral edema in experimental stroke

Autor: Raymond Chuen-Chung Chang, Kwok-Fai So, Di Yang, Amy C. Y. Lo, David Wong, Chung-Man Yeung, Suk-Yee Li
Rok vydání: 2011
Předmět:
Male
Pathology
Anatomy and Physiology
lcsh:Medicine
Brain Edema
chemistry.chemical_compound
Mice
Neuroprotective Agents - pharmacology
Edema
lcsh:Science
Stroke
Evans Blue
Multidisciplinary
Glial fibrillary acidic protein
biology
Infarction
Middle Cerebral Artery
Animal Models
Lycium
Stroke - complications - drug therapy - pathology - physiopathology
Extravasation
medicine.anatomical_structure
Neuroprotective Agents
Matrix Metalloproteinase 9
Neurology
Blood-Brain Barrier
Medicine
medicine.symptom
Research Article
medicine.medical_specialty
Drugs and Devices
Cerebrovascular Diseases
Brain Edema - etiology - pathology - physiopathology - prevention and control
Ischemia
Drugs
Chinese Herbal - pharmacology
Nerve Tissue Proteins
Blood–brain barrier
Neurological System
Cerebral edema
Model Organisms
Glial Fibrillary Acidic Protein
medicine
Animals
Biology
Aquaporin 4
business.industry
Plant Extracts
lcsh:R
medicine.disease
Blood-Brain Barrier - drug effects
Mice
Inbred C57BL
Disease Models
Animal
Oxidative Stress
chemistry
biology.protein
lcsh:Q
business
Drugs
Chinese Herbal
Phytotherapy
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 3, p e33596 (2012)
ISSN: 1932-6203
Popis: BACKGROUND AND PURPOSE: Ischemic stroke is a destructive cerebrovascular disease and a leading cause of death. Yet, no ideal neuroprotective agents are available, leaving prevention an attractive alternative. The extracts from the fruits of Lycium barbarum (LBP), a Chinese anti-aging medicine and food supplement, showed neuroprotective function in the retina when given prophylactically. We aim to evaluate the protective effects of LBP pre-treatment in an experimental stroke model. METHODS: C57BL/6N male mice were first fed with either vehicle (PBS) or LBP (1 or 10 mg/kg) daily for 7 days. Mice were then subjected to 2-hour transient middle cerebral artery occlusion (MCAO) by the intraluminal method followed by 22-hour reperfusion upon filament removal. Mice were evaluated for neurological deficits just before sacrifice. Brains were harvested for infarct size estimation, water content measurement, immunohistochemical analysis, and Western blot experiments. Evans blue (EB) extravasation was determined to assess blood-brain barrier (BBB) disruption after MCAO. RESULTS: LBP pre-treatment significantly improved neurological deficits as well as decreased infarct size, hemispheric swelling, and water content. Fewer apoptotic cells were identified in LBP-treated brains by TUNEL assay. Reduced EB extravasation, fewer IgG-leaky vessels, and up-regulation of occludin expression were also observed in LBP-treated brains. Moreover, immunoreactivity for aquaporin-4 and glial fibrillary acidic protein were significantly decreased in LBP-treated brains. CONCLUSIONS: Seven-day oral LBP pre-treatment effectively improved neurological deficits, decreased infarct size and cerebral edema as well as protected the brain from BBB disruption, aquaporin-4 up-regulation, and glial activation. The present study suggests that LBP may be used as a prophylactic neuroprotectant in patients at high risk for ischemic stroke.
published_or_final_version
Databáze: OpenAIRE
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