Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats

Autor: Shu-Cheng Zhang, Xue-Li Li, Yu-Zuo Bai, Wen-Kai Huang, Li-Na Yang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Cell type
Pathology
medicine.medical_specialty
Medicine (General)
Article Subject
Clinical Biochemistry
SOX10
Cell Culture Techniques
Nerve Tissue Proteins
Biology
03 medical and health sciences
0302 clinical medicine
R5-920
Neural Stem Cells
Cell Movement
Genetics
medicine
Animals
Paired Box Transcription Factors
Receptors
Growth Factor
Rats
Wistar
Molecular Biology
Cells
Cultured
Cell Nucleus
Paraffin Embedding
SOXE Transcription Factors
Biochemistry (medical)
Proto-Oncogene Proteins c-ret
Neural tube
Neural crest
General Medicine
Embryonic stem cell
Staining
Rats
030104 developmental biology
medicine.anatomical_structure
Neural Crest
030220 oncology & carcinogenesis
embryonic structures
Immunohistochemistry
Female
sense organs
Stem cell
Biomarkers
Research Article
Zdroj: Disease Markers
Disease Markers, Vol 2020 (2020)
ISSN: 1875-8630
0278-0240
Popis: Objective. Neural crest stem cells (NCSCs) are prototypically migratory cells immigrating from the dorsal neural tube to specific embryonic sites where they generate a variety of cell types. A lot of biomarkers for NCSCs have been identified. However, which biomarkers are the most specific is still unclear.Methods. The rat embryos harvested in embryonic day 9 (E9), E9.5, E10, E10.5, E11, E12, E13, and E14 were paraffin-embedded and sectioned in transverse. NCSCs were spatiotemporally demonstrated by immunohistochemical staining with RET, p75NTR, Pax7, and Sox10. NCSCs were isolated, cultured, and stained with RET, p75NTR, Pax7, and Sox10.Results. In the paraffin sections of rat embryos, the immunohistochemical staining of RET, p75NTR, and Sox10 can all be used in demonstrating NCSCs. Sox10 was positive mainly in NCSCs while RET and p75NTR were positive not only in NCSCs but also in other tissue cells. In primary culture cells, Sox10 was mainly in the nucleus of NCSCs, RET was mainly in the membrane, and p75NTR was positive in cytoplasm and membrane.Conclusions. Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect.
Databáze: OpenAIRE
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