Sox10 Is a Specific Biomarker for Neural Crest Stem Cells in Immunohistochemical Staining in Wistar Rats
Autor: | Shu-Cheng Zhang, Xue-Li Li, Yu-Zuo Bai, Wen-Kai Huang, Li-Na Yang |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Cell type Pathology medicine.medical_specialty Medicine (General) Article Subject Clinical Biochemistry SOX10 Cell Culture Techniques Nerve Tissue Proteins Biology 03 medical and health sciences 0302 clinical medicine R5-920 Neural Stem Cells Cell Movement Genetics medicine Animals Paired Box Transcription Factors Receptors Growth Factor Rats Wistar Molecular Biology Cells Cultured Cell Nucleus Paraffin Embedding SOXE Transcription Factors Biochemistry (medical) Proto-Oncogene Proteins c-ret Neural tube Neural crest General Medicine Embryonic stem cell Staining Rats 030104 developmental biology medicine.anatomical_structure Neural Crest 030220 oncology & carcinogenesis embryonic structures Immunohistochemistry Female sense organs Stem cell Biomarkers Research Article |
Zdroj: | Disease Markers Disease Markers, Vol 2020 (2020) |
ISSN: | 1875-8630 0278-0240 |
Popis: | Objective. Neural crest stem cells (NCSCs) are prototypically migratory cells immigrating from the dorsal neural tube to specific embryonic sites where they generate a variety of cell types. A lot of biomarkers for NCSCs have been identified. However, which biomarkers are the most specific is still unclear.Methods. The rat embryos harvested in embryonic day 9 (E9), E9.5, E10, E10.5, E11, E12, E13, and E14 were paraffin-embedded and sectioned in transverse. NCSCs were spatiotemporally demonstrated by immunohistochemical staining with RET, p75NTR, Pax7, and Sox10. NCSCs were isolated, cultured, and stained with RET, p75NTR, Pax7, and Sox10.Results. In the paraffin sections of rat embryos, the immunohistochemical staining of RET, p75NTR, and Sox10 can all be used in demonstrating NCSCs. Sox10 was positive mainly in NCSCs while RET and p75NTR were positive not only in NCSCs but also in other tissue cells. In primary culture cells, Sox10 was mainly in the nucleus of NCSCs, RET was mainly in the membrane, and p75NTR was positive in cytoplasm and membrane.Conclusions. Sox10 is the specific marker for immunohistochemical staining of NCSCs in paraffin sections. In cultured cells, Sox10, p75NTR, and RET presented a similar staining effect. |
Databáze: | OpenAIRE |
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