Nephroblastoma overexpressed gene (NOV) codes for a growth factor that induces protein tyrosine phosphorylation

Autor: Xin-Jun Liu, Paul D. Crowe, Errol B. De Souza, Frank Manu, Richard A. Maki, Nicholas Ling, Guy Barry, Gregory S. Kelner, Changlu Liu, Jun Fan
Rok vydání: 1999
Předmět:
DNA
Complementary
medicine.medical_treatment
Molecular Sequence Data
Biology
Wilms Tumor
Immediate early protein
Dexamethasone
Immediate-Early Proteins
chemistry.chemical_compound
Mice
Nephroblastoma Overexpressed Protein
stomatognathic system
Proto-Oncogene Proteins
Genetics
medicine
Tumor Cells
Cultured
Animals
Humans
Amino Acid Sequence
RNA
Messenger
Cloning
Molecular
Phosphorylation
Regulation of gene expression
Sequence Homology
Amino Acid
Growth factor
Connective Tissue Growth Factor
Tyrosine phosphorylation
General Medicine
Transfection
3T3 Cells
Oncogene Proteins
Viral
bacterial infections and mycoses
Molecular biology
Kidney Neoplasms
Recombinant Proteins
Rats
CTGF
Gene Expression Regulation
Neoplastic
chemistry
CYR61
Intercellular Signaling Peptides and Proteins
Tyrosine
Cell Division
Zdroj: Gene. 238(2)
ISSN: 0378-1119
Popis: NOV (nephroblastoma overexpressed gene) is a member of the CCN (connective tissue growth factor [CTGF], Cyr61/Cef10, NOV) family of proteins. These proteins are cysteine-rich and are noted for having growth-regulatory functions. We have isolated the rat NOV gene, and the DNA sequence shares 90% identity with the mouse and 80% identity with the human sequences. The rat NOV gene was expressed in all rat tissues examined, including brain, lung, heart, kidney, liver, spleen, thymus and skeletal muscle. Higher levels of rat NOV mRNA were seen in the brain, lung and skeletal muscle compared to the other tissues. Examination of NOV expression in various human cell lines revealed that NOV was expressed in U87, 293, T98G, SK-N-MC and Hs683 but not in HepG2, HL60, THP1 and Jurkat. The human NOV gene was transfected into 293 cells and the expressed protein purified. When 3T3 fibroblasts were treated with this recombinant NOV protein, a dose-dependent increase in proliferation was observed. Analysis of tyrosine-phosphorylated proteins revealed that when 3T3 cells were treated with NOV, a 221 kDa protein was phosphorylated. These data suggest that NOV can act as a growth factor for some cells and binds to a specific receptor that leads to the phosphorylation of a 221 kDa protein.
Databáze: OpenAIRE
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