Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea
| Autor: | John D. Mahon, Catherine DeLong, Shaun L. A. Hobbs, Maureen Anderson, Lawrence E. Pelcher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Oxygenase
Ribulose 1 5-bisphosphate biology fungi RuBisCO food and beverages Plant Science ribulose-1 5-bisphosphate carboxylase/oxygenase (variation) chemistry.chemical_compound Greening chemistry Biochemistry Complementary DNA parasitic diseases Gene expression Genetics biology.protein Genetic variability Pisum (rubisco variation) Gene greening and rubisco |
| DOI: | 10.1007/bf02411448 |
| Popis: | The amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) was studied during greening in 12Pisum sativum L. genotypes. The proportion of mRNA coding for the small subunit of Rubisco (SSU mRNA) was also monitored by hybridization with a cDNA (complementary DNA) probe from one of the nuclear genes coding for SSU (rbcS). Both the SSU mRNA and Rubisco (in g·FW)(-1)) contents rapidly increased in all genotypes on illumination of dark-grown seedlings. Natural genetic variability was found in the amounts of SSU mRNA, Rubisco· (g FW)(-1), total RNA·(g FW)(-1) and mRNA·(μg total RNA)(-1). Differences among genotypes in SSU mRNA were apparently the result of differences in the rate of light-induced accumulation of therbcS gene transcripts. Genotype means for SSU mRNA and Rubisco· (g FW)(-1) amounts during greening were significantly correlated (r=0.788;P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|