Osthole inhibits osteoclasts formation and bone resorption by regulating NF-κB signaling and NFATc1 activations stimulated by RANKL
Autor: | Lining Wang, Yalan Pan, Jiake Xu, Pengcheng Tu, Yang Guo, Suyang Zheng, Jie Sun, Yong Ma |
---|---|
Rok vydání: | 2018 |
Předmět: |
musculoskeletal diseases
0301 basic medicine Osteoclasts Stimulation Pharmacology Biochemistry Bone resorption 03 medical and health sciences Mice 0302 clinical medicine Osteoclast Coumarins medicine Animals Bone Resorption Receptor Molecular Biology Cathepsin biology NFATC Transcription Factors Activator (genetics) Chemistry RANK Ligand Acid phosphatase NF-kappa B Cell Differentiation Cell Biology 030104 developmental biology medicine.anatomical_structure RAW 264.7 Cells Gene Expression Regulation RANKL 030220 oncology & carcinogenesis biology.protein Signal Transduction |
Zdroj: | Journal of cellular biochemistry. 120(9) |
ISSN: | 1097-4644 |
Popis: | Chinese herbal medicine Fructus Cnidii has an outstanding effect on chronic lumbar pain and impotence, also has been used against osteoporosis with high frequency. Yet, the mechanisms of osthole, a derivative of Fructus Cnidii, on osteoclasts remains barely known. In this study, it was found out that osthole (10-6 mol/L, 10-5 mol/L) had the influence of inhibiting osteoclast formation and bone resorptive activities induced by receptor activator of nuclear factor κB ligand (RANKL), rather than affecting the viability of osteoclast-like cells. Furthermore, osthole could also inhibit the messenger RNA expressions of c-Src, tartrate-resistant acid phosphatase, β3-Integrin, matrix metallopeptidase 9, and cathepsin K. The results of the mechanistic study indicated that osthole regulated the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) and nuclear factor-κB (NF-κB) activations following the RANKL stimulation. These findings suggested that the inhibitory effects of osthole were associated with restraining the activations of NFATc1 and NF-κB induced by RANKL. Thus osthole can be used as a potential treatment for abnormal bone-resorption related diseases. |
Databáze: | OpenAIRE |
Externí odkaz: |