Molecular detection of Bartonella henselae and Bartonella clarridgeiae in clinical samples of pet cats from Southern Italy
| Autor: | E. La Camera, Maria Grazia Pennisi, Valeria Lentini, Sebastiana Zummo, M.T. Fera, B.M. Orlandella, L. Giacobbe |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Male Bartonella Pathology medicine.medical_specialty Polymerase Chain Reaction Bartonella clarridgeiae Nested-PCR Bartonella Infections medicine Animals Humans Lymph node Mouth Bartonella henselae CATS General Veterinary biology Bartonellosis Gene Amplification biology.organism_classification medicine.disease Virology Tick Infestations Oral swab–blood–lymph node samples medicine.anatomical_structure Italy Animals Domestic Cats Siphonaptera Female Lymph Nodes Lymph Bartonella Infection |
| Zdroj: | Research in Veterinary Science. 88:379-384 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/j.rvsc.2009.11.005 |
| Popis: | Bartonella henselae is considered an emerging pathogen of veterinary and medical interest that can be occasionally transmitted to humans. Cats are considered to be the only reservoir host for B. henselae. In this study, we used a nested-PCR assay to investigate the prevalence of B. henselae and Bartonella clarridgeiae DNA in peripheral blood samples, fine needle lymph node aspirate specimens and oral swabs from 85 cats in order to develop an easy diagnostic strategy for the selection of infection-free cats that are being considered as pets, especially for immunocompromised patients. Overall, molecular analysis showed that 71 cats (83.5%) tested PCR positive for the presence of B. henselae DNA. PCR amplification of DNA B. henselae produced positive products from lymph node aspirate specimens (62/85; 72.9%) similar to those obtained from blood samples (60/85; 70.6%) and higher than those from oral swabs (51/85; 60%) of cats. No PCR product was obtained for B. clarridgeiae. The simultaneous analysis of three different clinical samples in our study increased the diagnostic possibilities for B. henselae infection in the examined cats from 60–72.9% to 83.5%. Lymph node aspirates were found to be the most effective clinical samples for the detection of B. henselae and blood samples were the next best. Oral swab samples were used in this study with good results when considered in combination with blood and/or lymph node aspiration. The use of nested-PCR assay on these three clinical samples may enhance the diagnostic sensitivity for bartonellosis in cats irrespective of the clinical status of animals. |
| Databáze: | OpenAIRE |
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