Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter
| Autor: | Christian Bendixen, Jamal Momeni, Claus Hedegaard, Claus Oxvig, Carina Henriksen, Lone Bruhn Madsen, Knud Larsen, Kasper Kjaer-Sorensen |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Synapsin I
Transgene NRSE neuron restrictive silencer element RE1-silencing transcription factor Ab antibody GFP Article General Biochemistry Genetics and Molecular Biology Transgenic Green fluorescent protein R retina LLG lateral line ganglion REST RE1-silencing transcription factor Neuron-specific promoter FB forebrain Gene Zebrafish Chr chromosome GFP green fluorescent protein Genetics Pig biology FCO frontal cortex Nucleic acid sequence BST brain stem CMV cytomegalovirus biology.organism_classification ON olfactory neuron BSG basal ganglia Cell biology TG trigeminal ganglion MB midbrain OC optic chiasm HIP hippocampus Phosphoprotein biology.protein CBE cerebellum TSS transcription start site WPRE Woodchuck hepatitits virus Post-transcriptional Regulatory Element HB hindbrain |
| Zdroj: | Hedegaard, C, Kjaer-Sorensen, K, Madsen, L B, Henriksen, C, Momeni, J, Bendixen, C, Oxvig, C & Larsen, K E 2013, ' Porcine synapsin 1: SYN1 gene analysis and functional characterization of the promoter ', F E B S Open Bio, vol. 3, pp. 411–420 . https://doi.org/10.1016/j.fob.2013.10.002 FEBS Open Bio |
| DOI: | 10.1016/j.fob.2013.10.002 |
| Popis: | Synapsin 1 (SYN1) is a phosphoprotein involved in nerve signal transmission. The porcine SYN1 promoter orthologue was cloned and characterized to provide a means of expressing a transgene specifically in neurons. The nucleotide sequence of the promoter displayed a high degree of conservation of elements responsible for neuron-specific expression. Expression analysis of SYN1 demonstrated presence of transcript during embryonic development. Analysis of GFP expression in transgenic zebrafish embryos suggests that the pig SYN1 promoter directs expression in neuronal cells. Thus, the SYN1 promoter is a good candidate for use in the generation of pig models of human neurodegenerative disorders. Highlights • The porcine synapsin1 (SYN1) promoter was cloned and characterized. • SYN1 mRNA expression is detected in brain during embryo development. • The SYN1 gene is mapped to pig chromosome X. • Porcine SYN1 directs GFP expression in neuronal cells of transgenic zebrafish. |
| Databáze: | OpenAIRE |
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