An improved method for HLA-B and -C supratyping
| Autor: | Michela Mazzocco, Lucia Garbarino, Patrizia Larghero, Mauro S. Malnati, Francesca Sironi, M. Montera, Irene Vanni, Alessandro Raso, Roberto Biassoni, Francesco Broccolo, Elisabetta Ugolotti, E. Di Marco |
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| Přispěvatelé: | Biassoni, R, Malnati, M, Vanni, I, Raso, A, Sironi, F, Broccolo, F, Garbarino, L, Mazzocco, M, Montera, M, Larghero, P, Di Marco, E, Ugolotti, E |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Genotyping
T-Lymphocytes Immunology Histocompatibility Testing Human leukocyte antigen NK cells HLA-C Antigens Biology Receptors KIR Genotype Receptors HLA-B Antigens Humans Killer Cells Immunology and Allergy NK cell Allele Alleles Genetics HLA HLA-B (Bw4/Bw6) HLA-C (C1/C2) KIR Pyrosequencing Base Sequence High-Throughput Nucleotide Sequencing Killer Cells Natural Molecular Typing Sequence Analysis DNA HLA-C Antigen Repertoire DNA HLA-B HLA-B Antigen T-Lymphocyte Natural Sequence Analysis Human |
| Popis: | A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases. We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I(80), Bw4T(80), Bw6 and HLA-C1 or -C2 supratype. |
| Databáze: | OpenAIRE |
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