The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice
| Autor: | Shuji Yamano, Junko Komatsu, Akira Tokumura, Akira Kuwahara, Minoru Irahara |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pertussis toxin
General Biochemistry Genetics and Molecular Biology Mice chemistry.chemical_compound Lysophosphatidic acid Cyclic AMP medicine Animals Enzyme Inhibitors General Pharmacology Toxicology and Pharmaceutics Protein kinase A biology Oocyte activation General Medicine Oocyte Genistein Molecular biology Cell biology Meiosis medicine.anatomical_structure Pertussis Toxin chemistry Mitogen-activated protein kinase Oocytes biology.protein Female Lysophospholipids Signal transduction Intracellular Signal Transduction |
| Zdroj: | Life Sciences. 79:506-511 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2006.01.028 |
| Popis: | The signaling pathways linking to lysophosphatidic acid-promoted meiotic maturation in mice were studied. When mouse oocyte-cumulus cells complexes were cultured with 10(-5) M lysophosphatidic acid (the LPA group), the rate of oocyte nuclear maturation was significantly increased. Additions of pertussis toxin, genistein, U73122, Ro320432, PD98059 or SB203580 significantly suppressed the increase in lysophosphatidic acid-stimulated nuclear maturation rate. These results suggested that Gi/o-coupled lysophosphatidic acid receptors activate phosphatidylinositol-specific phospholipase C, and result in ERK and MAP kinase activation, which is triggered by diacylglycerol-dependent protein kinase C. When intracellular cAMP concentrations of oocytes in the LPA and control groups were measured using the acetylation assay, the intracellular cAMP concentration of an oocyte in the LPA group was significantly lower than the control oocyte (0.117+/-0.04 fmol/oocyte vs. 0.176+/-0.036 fmol/oocyte, p0.05). In conclusion, our results suggested that lysophosphatidic acid stimulates phospholipase C through a Gi-protein linked receptor on the surface of mouse cumulus cells and stimulates both extracellular signal-regulated kinase and p38 mitogen-activated kinase, resulting in the closure or loose of gap junctions between cumulus cells and the oocyte. The resultant early decrease of oocyte cAMP levels may promote nuclear maturation of mouse oocytes in vitro. |
| Databáze: | OpenAIRE |
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