Class I-restricted CTL induction by mucosal immunization with naked DNA encoding measles virus haemagglutinin
| Autor: | D Kaiserlian, R Buckland, N Etchart, M A Liu, T F Wild |
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| Rok vydání: | 1997 |
| Předmět: |
Cholera Toxin
Measles Vaccine Hemagglutinins Viral chemical and pharmacologic phenomena Biology law.invention DNA vaccination Measles virus Fatty Acids Monounsaturated Mice Plasmid Immunity law Virology Animals Immunity Mucosal Cells Cultured Mice Inbred BALB C Vaccines Synthetic Histocompatibility Antigens Class I Mouth Mucosa hemic and immune systems biology.organism_classification Cytotoxicity Tests Immunologic Quaternary Ammonium Compounds CTL Immunization Naked DNA Immunology DNA Viral Recombinant DNA T-Lymphocytes Cytotoxic |
| Zdroj: | The Journal of general virology. 78 |
| ISSN: | 0022-1317 |
| Popis: | We have investigated the class I-restricted CTL response specific for measles virus haemagglutinin (HA) in the spleens of mice immunized by various mucosal routes with a DNA plasmid carrying the HA gene (pV1j-HA). A single immunization with recombinant DNA injected in the buccal mucosa induced an HA-specific CTL response. Similarly, nasal immunization with the DNA vaccine induced primary CTLs against measles virus HA. Booster immunization did not enhance the CTL activity. Oral or intrajejunal immunization with the plasmid induced a CTL response of lower magnitude. However, this could be potentiated by co-administration of the mucosal adjuvant cholera toxin or cationic lipids (DOTAP). These data show that a CTL response can be generated by mucosal vaccination using DNA vaccines. |
| Databáze: | OpenAIRE |
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