Augmented anti-angiogenesis activity of polysulfated heparin-endostatin and polyethylene glycol-endostatin in alkali burn-induced corneal ulcers in rabbits
| Autor: | Zhao‑Na Li, Ya‑Li Zhang, Li‑Jun Cao, Ming Hu, Ming‑Xu Ge, Guo‑Ying Mu, Zhong‑Fang Yuan |
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| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
business.industry Angiogenesis Cell Articles General Medicine Pharmacology medicine.disease Angiogenesis inhibitor Endothelial stem cell Vascular endothelial growth factor chemistry.chemical_compound medicine.anatomical_structure Immunology and Microbiology (miscellaneous) chemistry In vivo Immunology Corneal neovascularization medicine Endostatin business |
| Zdroj: | Experimental and Therapeutic Medicine. 10:889-894 |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2015.2602 |
| Popis: | Endostatin (ES) is an endogenous angiogenesis inhibitor that has the ability to inhibit tumor growth and metastasis. However, its clinical application is limited by a number of disadvantages, such as poor stability, short half-life and the requirement of high doses to maintain its efficacy. The chemical modification on ES may offer a solution to these disadvantages. The aim of the present study was to evaluate the effects of ES, polysulfated heparin-endostatin (PSH-ES) and polyethylene glycol-endostatin (PEG-ES) on the endothelial cell proliferation and angiogenesis associated with corneal neovascularization (CNV) and to determine their mechanisms of action. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) was used to study the effects of ES and its derivatives on endothelial cell proliferation in vitro, and rabbits were used to evaluate the effects of ES and its derivatives on CNV in vivo. In the evaluation of CNV, the expression of vascular endothelial growth factor in the cornea was measured via immunohistochemistry and microvessels were counted. ES and its derivatives significantly inhibited endothelial cell proliferation in vitro (P |
| Databáze: | OpenAIRE |
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