A Rapid and Simple Detection Assay for Rice Bacterial Leaf Blight by Recombinase Polymerase Amplification
| Autor: | Soo Yeon Choi, Bong Choon Lee, Sang-Min Kim, Shinhwa Kim, Hyun-Ju Kim, Su Jwa Seo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
bacterial leaf blight
fungi detection food and beverages Recombinase Polymerase Amplification Plant Science Biology xanthomonas oryzae pv. oryzae lcsh:S1-972 Biochemistry Molecular biology Blight recombinase polymerase amplification lcsh:Agriculture (General) Agronomy and Crop Science Molecular Biology Biotechnology |
| Zdroj: | Research in Plant Disease, Vol 26, Iss 4, Pp 195-201 (2020) |
| ISSN: | 2233-9191 1598-2262 |
| DOI: | 10.5423/rpd.2020.26.4.195 |
| Popis: | Rice bacterial leaf blight (BLB) by Xanthomonas oryzae pv. oryzae (Xoo) is considered to be one of the major rice diseases steadily occurring around the rice-producing countries. In this study, we developed a recombinase polymerase amplification (RPA) assay for the rapid, convenient and specific diagnosis of Xoo by targeting Xoo-specific transposase A gene. As the target gene can be amplified in 10 min without DNA extraction process and special equipment for temperature control, RPA for BLB can be useful and practical component for on-site diagnosis. |
| Databáze: | OpenAIRE |
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