Bleomycin induced inhibition of dna synthesis in ataxia-telangiectasia cell lines
| Autor: | Alexander M. R. Taylor, Malcolm J. Edwards, E.J. Flude |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
DNA Replication
Biophysics Biology Bleomycin Biochemistry Cell Line Ataxia Telangiectasia chemistry.chemical_compound medicine Humans Replicon Fibroblast Molecular Biology DNA synthesis Lymphoblast Homozygote DNA replication DNA Cell Biology Molecular biology Kinetics medicine.anatomical_structure chemistry Cell culture |
| Zdroj: | Biochemical and Biophysical Research Communications. 102:610-616 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(81)80175-1 |
| Popis: | Summary Exposure of fibroblast and lymphoblastoid cell lines from normal subjects to bleomycin (30–300 μg/ml) results in a dose dependent biphasic inhibition of DNA synthesis when compared with untreated cells. Using alkaline sucrose density gradient sedimentation we have confirmed that bleomycin decreases both DNA replicon initiation and chain elongation in normal lymphoblastoid cells. Doses of up to 100 μ/ml which caused a marked decrease in the rate of DNA synthesis in normal lymphoblastoid cells had much less effect on the inhibition of DNA synthesis in lymphoblastoid cell lines from ataxia-telangiectasia patients. The inhibition of DNA replicon initiation and chain elongation in these cell lines occurred only at high doses of bleomycin (100 μg/ml). |
| Databáze: | OpenAIRE |
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