Arabidopsis thaliana plastidial methionine sulfoxide reductases B, MSRBs, account for most leaf peptide MSR activity and are essential for growth under environmental constraints through a role in the preservation of photosystem antennae

Autor: Françoise Eymery, Lionel Tarrago, Christina Vieira Dos Santos, Pascal Rey, Edith Laugier, Michel Havaux
Přispěvatelé: CEA Cadarache, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Biodiversité et Biotechnologie Fongiques (BBF), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Chlorophyll
0106 biological sciences
Chloroplasts
Genotype
Light
Blotting
Western
Arabidopsis
Plant Science
01 natural sciences
Substrate Specificity
03 medical and health sciences
chemistry.chemical_compound
Methionine
Genetics
Arabidopsis thaliana
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Photosynthesis
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Photosystem
0303 health sciences
biology
Arabidopsis Proteins
Methionine sulfoxide
Temperature
food and beverages
Cell Biology
Plants
Genetically Modified
biology.organism_classification
Adaptation
Physiological
Isoenzymes
Plant Leaves
Chloroplast
Phenotype
Biochemistry
chemistry
Methionine Sulfoxide Reductases
Thylakoid
Mutation
Methionine sulfoxide reductase
010606 plant biology & botany
Zdroj: Plant Journal
Plant Journal, 2010, 61 (2), pp.271-282. ⟨10.1111/j.1365-313X.2009.04053.x⟩
Plant Journal, Wiley, 2010, 61 (2), pp.271-282. ⟨10.1111/j.1365-313X.2009.04053.x⟩
ISSN: 0960-7412
1365-313X
DOI: 10.1111/j.1365-313X.2009.04053.x⟩
Popis: Methionine oxidation to methionine sulfoxide (MetSO) is reversed by two types of methionine sulfoxide reductases (MSRs), A and B, specific to MetSO S- and R-diastereomers, respectively. Two MSRB isoforms, MSRB1 and MSRB2, are present in chloroplasts of Arabidopsis thaliana. To assess their physiological role, we characterized Arabidopsis mutants knockout for the expression of MSRB1, MSRB2 or both genes. Measurements of MSR activity in leaf extracts revealed that the two plastidial MSRB enzymes account for the major part of leaf peptide MSR capacity. Under standard conditions of light and temperature, plants lacking one or both plastidial MSRBs do not exhibit any phenotype, regarding growth and development. In contrast, we observed that the concomitant absence of both proteins results in a reduced growth for plants cultivated under high light or low temperature. In contrast, double mutant lines restored for MSRB2 expression display no phenotype. Under environmental constraints, the MetSO level in leaf proteins is higher in plants lacking both plastidial MSRBs than in Wt plants. The absence of plastidial MSRBs is associated with an increased chlorophyll a/b ratio, a reduced content of Lhca1 and Lhcb1 proteins and an impaired photosynthetic performance. Finally, we show that MSRBs are able to use as substrates, oxidized cpSRP43 and cpSRP54, the two main components involved in the targeting of Lhc proteins to the thylakoids. We propose that plastidial MSRBs fulfil an essential function in maintaining vegetative growth of plants during environmental constraints, through a role in the preservation of photosynthetic antennae.
Databáze: OpenAIRE
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