Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature
| Autor: | M. Vendrell, Eva Domı́nguez-Puigjaner, Immaculada Llop-Tous |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Complementary Physiology Sequence analysis Molecular Sequence Data Plant Science Biology Fragaria Gene Expression Regulation Enzymologic Gene Expression Regulation Plant Gene expression Amino Acid Sequence Cloning Molecular Protein kinase A Regulation of gene expression Sequence Homology Amino Acid cDNA library Gene Expression Regulation Developmental food and beverages Ripening Sequence Analysis DNA Cold Temperature Biochemistry Protein kinase domain Regulatory sequence Fruit Protein Kinases |
| Zdroj: | Journal of Experimental Botany. 53:2283-2285 |
| ISSN: | 1460-2431 |
| Popis: | A full-length cDNA clone (FaCDPK1) encoding a calcium-dependent protein kinase (CDPK) has been isolated from a strawberry fruit cDNA library. FaCDPK1 contains the basic features of CDPKs: a catalytic kinase domain linked to a regulatory calmodulin-like domain by a junction sequence that has been shown to act as an autoinhibitory pseudosubstrate. Although the calmodulin-like domain of CDPKs typically contains four EF-hand calcium-binding motifs, FaCDPK1 was predicted to contain only three EF-hand motifs. FaCDPK1 gene expression was observed in roots, stolons, meristems, flowers, and leaves. FaCDPK1 mRNA was not detected in young fruits, but accumulated as fruit turned to white, suggesting a role for this gene in the developing strawberry fruit. In ripe fruit the levels of transcript increased in response to low temperature. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|