Determination of nifurtimox in dog plasma by stable-isotope dilution LC–MS/MS
| Autor: | Mark-Jean Gnoth, Peter-Michael Hopfe, Uwe Thuss |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Clinical Biochemistry Indicator Dilution Techniques Isotope dilution Bioinformatics High-performance liquid chromatography Analytical Chemistry Plasma Dogs Limit of Detection Tandem Mass Spectrometry medicine Animals Protein precipitation Sample preparation General Pharmacology Toxicology and Pharmaceutics Nifurtimox Detection limit Reproducibility Chromatography Chemistry General Medicine Trypanocidal Agents Triple quadrupole mass spectrometer Medical Laboratory Technology Isotope Labeling Female Chromatography Liquid medicine.drug |
| Zdroj: | Bioanalysis. 7:2777-2787 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: Nifurtimox is a 5-nitrofuran derived antiprotozoal drug used to treat diseases caused by trypanosomes including Chagas’ disease and sleeping sickness (African trypanosomiasis). Available methods for the determination of nifurtimox in plasma are tedious and of low sensitivity. For the first time, an isotope dilution HPLC/MS/MS method for the sensitive quantitation of nifurtimox down to 10.0 μg/l in plasma is described. Results: Protein precipitation was used for sample preparation. Samples were analyzed on a standard triple quadrupole tandem mass spectrometer. The validated concentration range covers 10.0 μg/l (LLOQ) to 5000 μg/l. Interassay accuracy and precision (%CV) ranged from 98.4 to 101%, and 2.61 to 10.1%, respectively. Conclusion: The method consists of very simple sample preparation and provides unmatched sensitivity, high reproducibility and robustness enabling analysis of large sample numbers. Method performance met current guidelines on bioanalytical method validation. |
| Databáze: | OpenAIRE |
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