Sparger design as key parameter to define shear conditions in pneumatic bioreactors
| Autor: | Rodrigo Béttega, Marcel Otavio Cerri, Mateus N. Esperança, Guilherme Y. Rodriguez, Alberto C. Badino, Caroline E. Mendes |
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| Přispěvatelé: | Fed Inst Educ Sci & Technol Sao Paulo, Fed Univ Rio Grande, Univ Fed Itajuba, Universidade Estadual Paulista (Unesp), Universidade Federal de São Carlos (UFSCar) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Environmental Engineering Materials science Shear rate Pneumatic bioreactor Biomedical Engineering Bioengineering Computational fluid dynamics 01 natural sciences 03 medical and health sciences 010608 biotechnology Bioreactor Sparging 030304 developmental biology 0303 health sciences business.industry Airlift Mechanics Shear (geology) Performance comparison Sparger design business Order of magnitude Biotechnology |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1336-0000 |
| Popis: | Made available in DSpace on 2020-12-10T17:30:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-15 Human Resources Program of the Brazilian National Agency of Petroleum, Natural Gas, and Biofuels Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The average shear rate ((av)) is a parameter used to characterize the shear environment in bioreactors, enabling comparison of the performances of different bioreactor models in terms of microorganism morphology and viability, and consequently bioproduct formation. Based on this approach, pneumatic bioreactors have been classified as low shear devices. However, the shear behavior cannot be generalized over a wide range of operating conditions, suggesting that the maximum shear rate ((max)) may be more suitable for the purpose of bioreactor performance comparison. Therefore, the aim of this work was to evaluate average and maximum shear rates in pneumatic bioreactors (bubble column and airlift), based on computational fluid dynamics (CFD) simulations. Concentric-duct and split airlift bioreactors exhibited higher (av) values, compared to the bubble column design, due to the nature of the liquid circulation patterns. The pneumatic bioreactors exhibited a significant (order of magnitude) difference between (av) (11.0-27.3 s(-1)) and (max) 4555 to 25,040 s(-1)), reflecting a non-uniform spatial distribution. The (max) values occurred close to the sparger holes and presented a linear relationship with gas injection velocity, which is dependent on the sparger geometry. In this way, sparger characteristics (number and diameter of sparger holes) defined (max) values in pneumatic bioreactors, showing that sparger should be properly designed in order to avoid excessive local shear rates. Fed Inst Educ Sci & Technol Sao Paulo, Campus Capivari, BR-13360000 Capivari, SP, Brazil Fed Univ Rio Grande, Chem & Food Sch, Carreiros, RS, Brazil Univ Fed Itajuba, Nat Resources Inst, Bioproc Engn Course, Itajuba, MG, Brazil Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP, Brazil Univ Fed Sao Carlos, Grad Program Chem Engn, CP 676, BR-13565905 Sao Carlos, SP, Brazil Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara, SP, Brazil Human Resources Program of the Brazilian National Agency of Petroleum, Natural Gas, and Biofuels: PRH/ANP-44 CNPq: 478472/2011-0 FAPESP: 2011/23807-1 FAPESP: 2012/17756-8 CAPES: 001 |
| Databáze: | OpenAIRE |
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