Down-Regulation of MiR-30c Promotes the Invasion of Non-Small Cell Lung Cancer by Targeting MTA1
| Autor: | Yijiang Chen, Chen Wu, Qiyou Chen, Yang Xia, Bin Liu, Zhaopeng Zhong, Caihua Xu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Physiology
Blotting Western Down-Regulation Apoptosis Biology NSCLC Polymerase Chain Reaction lcsh:Physiology Histone Deacetylases MicroRNA-30c lcsh:Biochemistry Invasion Western blot Downregulation and upregulation Cell Movement Carcinoma Non-Small-Cell Lung Cell Line Tumor microRNA medicine Humans lcsh:QD415-436 Neoplasm Invasiveness Luciferase Lung cancer Cells Cultured Cell Proliferation A549 cell Gene knockdown Messenger RNA lcsh:QP1-981 medicine.diagnostic_test medicine.disease Immunohistochemistry respiratory tract diseases Repressor Proteins MicroRNAs MTA1 Trans-Activators Cancer research |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 32, Iss 2, Pp 476-485 (2013) |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000354452 |
| Popis: | Background: The connection between microRNA expression and lung cancer development has been identified in recent literature. However, the mechanism of microRNA has been poorly elucidated in non-small-cell lung cancer (NSCLC). Methods and Results: Comparing with adjacent tissues (n=75), miR-30c has a lower expression in lung cancer specimens (n=75). The knockdown of miR-30c enhanced the invasion of A549 cells; meanwhile, the overexpression of miR-30c could reverse the effect of the knockdown of miR-30c in vitro. A luciferase assay revealed that miR-30c was directly bound to the 3‘-untranslated regions (3‘-UTR) of MTA1. QRT-PCR and western blot shows MTA1 was up-regulated in mRNA and protein levels. The effect taken on the invasion of NSCLC by overexpression of MTA1 works the same as down-regulated miR-30c. Conclusion: miR-30c may play a pivotal role in controlling lung cancer invasion through regulating MTA1in NSCLC. |
| Databáze: | OpenAIRE |
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