Detection of MAPK/ERK pathway proteins and KRAS mutations in adenomatoid odontogenic tumors
| Autor: | Ronell Bologna-Molina, Celeste Sánchez-Romero, Ikuko Ogawa, Yasunori Takeda, Takashi Takata, Adalberto Mosqueda-Taylor, Toshinari Mikami, Mariana Villarroel-Dorrego |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Proto-Oncogene Proteins B-raf MAPK/ERK pathway Stromal cell Adolescent endocrine system diseases MAP Kinase Signaling System Biology medicine.disease_cause Ameloblastoma Proto-Oncogene Proteins p21(ras) Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans Missense mutation Child Extracellular Signal-Regulated MAP Kinases neoplasms General Dentistry Gene Adenomatoid odontogenic tumor 030206 dentistry Middle Aged medicine.disease digestive system diseases ErbB Receptors Proto-Oncogene Proteins c-raf Otorhinolaryngology Child Preschool 030220 oncology & carcinogenesis Cancer research Immunohistochemistry Female KRAS Carcinogenesis |
| Zdroj: | Oral Diseases. 25:481-487 |
| ISSN: | 1354-523X |
| DOI: | 10.1111/odi.12989 |
| Popis: | Objective This study aimed to assess the frequency of KRAS mutation and its association with the presence of the MAPK/ERK signaling pathway proteins in adenomatoid odontogenic tumors. Study design Paraffin-embedded tissue samples from nine cases of adenomatoid odontogenic tumor were used. Genomic DNA was extracted from each sample; in one case, genetic mutations in 50 cancer-associated genes were examined by next-generation sequencing. Hotspot mutations in the RAS family were analyzed by Luminex assay using the remaining eight cases. Subsequently, immunohistochemistry for KRAS, CRAF, BRAF, EGFR, ERK, MEK, and BRAFV600E was performed. Results A KRAS G12D missense mutation was detected in the DNA sequence of the tumor cells, but it was not detected in the stromal tissue. KRAS G12V and KRAS G12R mutations were detected in two and four cases, respectively. For immunohistochemistry, all the cases were EGFR, KRAS, BRAF, CRAF positive, one case was ERK negative,and one case was MEK and ERK negative, all the other remaining cases were MEK and ERK positive. Conclusion KRAS mutation at codon 12 and the presence of MAPK/ERK pathway proteins were detected suggesting their association with tumorigenesis of adenomatoid odontogenic tumors. |
| Databáze: | OpenAIRE |
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