The testis anion transporter 1 (Slc26a8) is required for sperm terminal differentiation and male fertility in the mouse
| Autor: | David L'Hôte, Jan A. Gossen, Aminata Touré, Pierre Lhuillier, Bernard Jégou, Gérard Gacon, Denise Escalier, Cor W. Kuil |
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| Přispěvatelé: | Institut Cochin ( UMR_S567 / UMR 8104 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes ( GERHM ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -IFR140-Institut National de la Santé et de la Recherche Médicale ( INSERM ), CNRS, INSERM, Université René Descartes, European Community (6th FPRTDD, ERG FP6-006313), 'Fonds d'Aide à la Recherche Organon' (FARO, Organon-France), Institut Cochin (UMR_S567 / UMR 8104), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-IFR140-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Male
Cellular differentiation Testicle MESH: Mice Knockout Germline Antiporters Mice 0302 clinical medicine Adenosine Triphosphate MESH : Antiporters MESH : Fertility Testis MESH: Adenosine Triphosphate MESH: Animals MESH : Adenosine Triphosphate MESH : Female Sperm annulus Genetics (clinical) Sperm motility MESH: Amino Acid Transport Systems Neutral Mice Knockout 0303 health sciences MESH: Testis MESH: Spermatozoa Cell Differentiation General Medicine MESH: Sperm Motility MESH: Anion Transport Proteins Spermatozoa Cell biology medicine.anatomical_structure MESH : Amino Acid Transport Systems Neutral Flagella Sulfate Transporters 030220 oncology & carcinogenesis Sperm Motility Female MESH : Mutation Acrosome MESH : Cell Differentiation MESH: Cell Differentiation medicine.medical_specialty MESH: Mutation MESH : Male Anion Transport Proteins Motility MESH : Mice Inbred C57BL Biology MESH: Acrosome MESH: Flagella 03 medical and health sciences TESTIS ANION TRANSPORTER 1 MESH: Mice Inbred C57BL Internal medicine MESH : Mice Genetics medicine MESH : Spermatozoa Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology MESH: Mice [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology 030304 developmental biology MESH : Testis MESH: Fertility Sperm MESH: Male Mice Inbred C57BL MESH : Sperm Motility Endocrinology Amino Acid Transport Systems Neutral Fertility MESH : Anion Transport Proteins Mutation MESH : Mice Knockout MESH: Antiporters MESH : Animals MESH : Acrosome MESH : Flagella MESH: Female |
| Zdroj: | Human Molecular Genetics Human Molecular Genetics, Oxford University Press (OUP), 2007, 16 (15), pp.1783-93. 〈10.1093/hmg/ddm117〉 Human Molecular Genetics, Oxford University Press (OUP), 2007, 16 (15), pp.1783-93. ⟨10.1093/hmg/ddm117⟩ Human Molecular Genetics, 2007, 16 (15), pp.1783-93. ⟨10.1093/hmg/ddm117⟩ |
| ISSN: | 0964-6906 1460-2083 |
| Popis: | International audience; The Slc26 family is a conserved family of anion transporters. In the human, their physiological relevance was highlighted with the discovery of pathogenic mutations in several Slc26 transporters that lead to distinctive clinical disorders (Pendred syndrome, deafness, diastrophic dysplasia, congenital chloride diarrhoea) that are related to the specific distribution of these genes. We previously identified TAT1 as a new family member (Slc26A8), very specifically expressed in male germ cells in both the human and the mouse. To investigate Tat1 function in the male germline, we generated mice with a targeted disruption of the Tat1 gene. Heterozygous and homozygous Tat1 mutant mice were indistinguishable from wild-type littermates concerning survival rate, general appearance and gross behaviour; however, Tat1 null males were sterile due to complete lack of sperm motility and reduced sperm fertilization potential. Ultra-structural analysis revealed defects in flagellar differentiation leading to an abnormal annulus, disorganization of the midpiece-principal piece junction, hairpin bending of the sperm tail with disruption of the axial structures, and abnormal mitochondrial sheath assembly. While ATP levels were normal, ATP consumption was strongly reduced in Tat1 null spermatozoa. Interestingly, Tat1 is located at the annulus, a septin-based circular structure connecting the midpiece to the principal piece. Altogether, our results indicate that Tat1 is a critical component of the sperm annulus that is essential for proper sperm tail differentiation and motility. |
| Databáze: | OpenAIRE |
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