NMR metabolic fingerprints of murine melanocyte and melanoma cell lines: application to biomarker discovery
| Autor: | Daniel Suss Riter, Guilherme L. Sassaki, Andersson Barison, Marcello Iacomini, Sheila M.B. Winnischofer, Arquimedes Paixão Santana-Filho, Thiago Jacomasso |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Melanoma Experimental Melanocyte Article 03 medical and health sciences Mice Metabolomics Cell Line Tumor medicine Metabolome Animals Biomarker discovery Principal Component Analysis Multidisciplinary Chemistry Melanoma Nuclear magnetic resonance spectroscopy medicine.disease Cell biology 030104 developmental biology medicine.anatomical_structure Biochemistry Gene Expression Regulation Cell culture Multivariate Analysis Melanocytes Tetradecanoylphorbol Acetate Heteronuclear single quantum coherence spectroscopy Biomarkers |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Melanoma is the most aggressive type of skin cancer and efforts to improve the diagnosis of this neoplasia are largely based on the use of cell lines. Metabolomics is currently undergoing great advancements towards its use to screening for disease biomarkers. Although NMR metabolomics includes both 1D and 2D methodologies, there is a lack of data in the literature regarding heteronuclear 2D NMR assignments of the metabolome from eukaryotic cell lines. The present study applied NMR-based metabolomics strategies to characterize aqueous and lipid extracts from murine melanocytes and melanoma cell lines with distinct tumorigenic potential, successfully obtaining fingerprints of the metabolites from the extracts of the cell lines by means of 2D NMR HSQC correlation maps. Relative amounts of the identified metabolites were compared between the 4 cell lines. Multivariate analysis of 1H NMR data was able not only to differentiate the melanocyte cell line from the tumorigenic ones but also distinguish among the 3 tumorigenic cell lines. We also investigated the effects of mitogenic agents, and found that they can markedly influence the metabolome of the melanocyte cell line, resembling the pattern of most proliferative cell lines. |
| Databáze: | OpenAIRE |
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