An ABC transporter encoding gene lndW confers resistance to landomycin E
| Autor: | Iryna Ostash, Victor Fedorenko, Bohdan Ostash, Anton Kobylyanskyy, Tatsunosuke Nakamura, Maksym Myronovskyy, Yuriy Rebets, Suzanne Walker |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Streptomyces globisporus Sequence analysis Gene Expression ATP-binding cassette transporter Microbial Sensitivity Tests Biology Biochemistry Microbiology Gene product chemistry.chemical_compound Open Reading Frames Bacterial Proteins Gene cluster Drug Resistance Bacterial Genetics Cloning Molecular Molecular Biology Gene General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology Streptomyces Anti-Bacterial Agents Open reading frame Mutagenesis Insertional Aminoglycosides chemistry Genes Bacterial Multigene Family ATP-Binding Cassette Transporters DNA Plasmids |
| Zdroj: | Archives of microbiology. 190(1) |
| ISSN: | 0302-8933 |
| Popis: | Streptomyces globisporus 1912 produces a polyketide antibiotic landomycin E (LaE), which possesses anticancer activity. A 1.8 kb DNA fragment at the end of landomycin E biosynthetic gene cluster was sequenced. DNA sequence analysis of this fragment identified one complete open reading frame, designated lndW. The deduced sequence of lndW gene product revealed significant similarity to the ATP-binding domains of the ABC (ATP-binding protein cassette) superfamily of transport-related proteins. Knockout of lndW had no significant effect on resistance to LaE and its production. The expression of lndW in S. globisporus 1912 was proven via transcriptional fusion of lndW promoter to EGFP (enhanced green fluorescent protein). Overexpression of lndW in S. lividans TK24 conferred resistance to LaE. The mechanism of lndW function in LaE biosynthesis is discussed. |
| Databáze: | OpenAIRE |
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