Effect of dietary calcium and 1,25-(OH)2D3 on the expression of calcium transport genes in calbindin-D9k and -D28k double knockout mice
| Autor: | Eui-Bae Jeung, Sang Hwan Ko, Goo Taeg Oh, Kyung Chul Choi |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Calbindins TRPV6 Normal diet Calcitriol TRPV5 Biophysics chemistry.chemical_element Gene Expression TRPV Cation Channels Nerve Tissue Proteins Calcium Biology Kidney Biochemistry Calbindin Bone and Bones Mice S100 Calcium Binding Protein G Bone Density Internal medicine medicine Animals Intestinal Mucosa Molecular Biology Bone mineral Calcium metabolism Mice Knockout Ion Transport Body Weight Calcium-Binding Proteins Cell Biology Calcium Dietary Endocrinology chemistry Calcium Channels Gene Deletion medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 379(2) |
| ISSN: | 1090-2104 |
| Popis: | The phenotypes of calbindin-D9k (CaBP-9k) and -28k (CaBP-28k) single knockout (KO) mice are similar to wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we generated CaBP-9k/CaBP-28k double knockout (DKO) mice in order to investigate the importance of CaBP-9k and CaBP-28k in active calcium processing. Under normal dietary conditions, DKO mice did not exhibit any changes in phenotype or the expression of active calcium transport genes as compared to WT or CaBP-28k KO mice. Under calcium-deficient dietary conditions, the phenotype and expression of calcium transport genes in CaBP-28k KO mice were similar to WT, whereas in DKO mice, serum calcium levels and bone length were decreased. The intestinal and renal expression of transient receptor potential vanilloid member 6 (TRPV6) mRNA was significantly decreased in DKO mice fed a calcium-deficient diet as compared to CaBP-28k KO or WT mice, and DKO mice died after 4 weeks on a calcium-deficient diet. Body weight, bone mineral density (BMD) and bone length were significantly reduced in all mice fed a calcium and 1,25-(OH)2D3-deficient diet, as compared to a normal diet, and none of the mice survived more than 4 weeks. These results indicate that deletion of CaBP-28k alone does not affect body calcium homeostasis, but that deletion of CaBP-9k and CaBP-28k has a significant effect on calcium processing under calcium-deficient conditions, confirming the importance of dietary calcium and 1,25-(OH)2D3 during growth and development. |
| Databáze: | OpenAIRE |
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