Targeting of ricin A chain into pea chloroplasts
| Autor: | Lynne M. Roberts, Julie W. Meadows, Colin Robinson |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Retargeting
Chloroplasts Recombinant Fusion Proteins Genetic Vectors Molecular Sequence Data Biophysics Ricin macromolecular substances Biology Genes Plant environment and public health Biochemistry chemistry.chemical_compound Ricin. Chloroplast Structural Biology polycyclic compounds Genetics Protein biosynthesis Amino Acid Sequence Molecular Biology Gene Triticum Plants Medicinal Protein transport Genetic transfer food and beverages Biological Transport Fabaceae Cell Biology Fusion protein Transport protein Molecular Weight Chloroplast chemistry Thylakoid lipids (amino acids peptides and proteins) Plant Lectins Protein Processing Post-Translational |
| Zdroj: | FEBS Letters. 293:134-136 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(91)81169-9 |
| Popis: | Achimaeric gene was constructed encoding the pre-sequence of the 33 kDa oxygen-evolving complex protein from wheat (a thylakoid lumen protein) linked to ricin A chain. The fusion protein is efficiently imported by isolated pea chloroplasts and localised partly in the stroma, with the remainder bound to the stromal surface of the thylakoids. The imported protein is fully processed by both the stromal and thylakoidal processing peptidases, indicating that partial or complete translocation across the thylakoid membrane has taken place. |
| Databáze: | OpenAIRE |
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