Effect of PCL/PEG-Based Membranes on Actinorhodin Production in Streptomyces coelicolor Cultivations
| Autor: | Alberto Sutera, Luigi Botta, Roberto Scaffaro, RM Fontana, Giuseppe Gallo, Anna Maria Puglia, Francesco Lopresti |
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| Přispěvatelé: | Scaffaro, R., Lopresti, F., Sutera, A., Botta, L., Fontana, R., Puglia, A., Gallo, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials Chemistry2506 Metals and Alloys
PCL/PEG membrane Polymers and Plastics Polyesters Particulate leaching S. coelicolor immobilization Anthraquinones Streptomyces coelicolor Bioengineering 02 engineering and technology Polyethylene glycol engineering.material 010402 general chemistry 01 natural sciences Actinorhodin Polyethylene Glycols Biomaterials chemistry.chemical_compound Melt mixing PEG ratio Botany Materials Chemistry Cell Aggregation Polymers and Plastic biology Chemistry Actinorhodin production technology industry and agriculture 021001 nanoscience & nanotechnology biology.organism_classification Biomaterial Cell aggregation Anti-Bacterial Agents 0104 chemical sciences Blue colored Membrane Chemical engineering Fermentation engineering Biopolymer 0210 nano-technology Biotechnology |
| Popis: | The actinomycetes, Gram-positive filamentous bacteria, are the most prolific source of natural occurring antibiotics. At an industrial level, antibiotics from actinomycete strains are produced by means of submerged fermentations, where one of the major factors negatively affecting bioproductivity is the pellet-shaped biomass growth. The immobilization of microorganisms on properly chosen supports prevents cell-cell aggregation resulting in improving the biosynthetic capability. Thus, novel porous biopolymer-based devices are developed by combining melt mixing and particulate leaching. In particular, polycaprolactone (PCL), polyethylene glycol (PEG), and sodium chloride (NaCl) with different grain sizes are used to prepare PCL/PEG/NaCl blends in the melt. These blends are then leached to obtain PCL-based porous membranes that are used as solid support for the growth of Streptomyces coelicolor, a model streptomycete used to produce various antibiotics including the blue colored actinorhodin (ACT). Thereafter, the effect of the devices' characteristics on the bacterial growth and on the production ACT is evaluated. The results showed that ACT production is strongly dependent on the pore size distribution of the device. Moreover, membranes with pores ranging from 90 to 110 μm are able to offer a potential improvement in volumetric productivity of ACT if compared to conventional submerged liquid culture. |
| Databáze: | OpenAIRE |
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