Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms

Autor: WM Silva, K.F. Lima, Cintia Junia Monteiro, P.M. Santos, Alessandra Rodrigues da Silva, Kumiko Koibuchi Sakane, Karen C. M. Moraes
Přispěvatelé: Univ Vale Paraiba, Universidade Federal de Ouro Preto (UFOP), Universidade Estadual Paulista (Unesp)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Time Factors
Physiology
Cellular homeostasis
Pharmacology
Biochemistry
H9c2 cardiac cell line
General Pharmacology
Toxicology and Pharmaceutics
Prostaglandin E2
lcsh:QH301-705.5
lcsh:R5-920
Sulfonamides
Spectroscopy
Near-Infrared
medicine.diagnostic_test
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Cyclooxygenase-2 inhibitor
General Medicine
Cellular and molecular analyses
COX-2 inhibitor
lcsh:Medicine (General)
Myoblasts
Cardiac
medicine.drug
Cell death
Programmed cell death
medicine.medical_specialty
Cell Survival
Blotting
Western
Immunology
Biophysics
Ocean Engineering
Cell Line
Western blot
Downregulation and upregulation
medicine
Animals
RNA
Messenger
Viability assay
Cell Proliferation
Cyclooxygenase 2 Inhibitors
Dose-Response Relationship
Drug
business.industry
Biomedical Sciences
Cell Biology
Rats
Surgery
lcsh:Biology (General)
Gene Expression Regulation
Celecoxib
Pyrazoles
business
Zdroj: Brazilian Journal of Medical and Biological Research v.47 n.1 2014
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Brazilian Journal of Medical and Biological Research, Volume: 47, Issue: 1, Pages: 50-59, Published: 29 NOV 2013
Brazilian Journal of Medical and Biological Research, Vol 47, Iss 1, Pp 50-59 (2014)
ISSN: 0100-879X
Popis: Made available in DSpace on 2014-12-03T13:10:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-01-01Bitstream added on 2014-12-03T13:22:51Z : No. of bitstreams: 1 S0100-879X2013005003028.pdf: 1194270 bytes, checksum: 67336b43240384274afa534b97906d9f (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Fundacao Valeparaibana de Ensino Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival. Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, Brazil Univ Fed Ouro Preto, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, Brazil Univ Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil Univ Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil CNPq: 475586/2009-3 CNPq: 506991/2010-5 FAPEMIG: 02351-10
Databáze: OpenAIRE
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