Twist1 Influences the Expression of Leading Members of the IL-17 Signaling Pathway in HER2-Positive Breast Cancer Cells
| Autor: | Bárbara Du Rocher, Eliana Abdelhay, Daniel Bulzico, Stephany Corrêa, Everton Cruz dos Santos, Luize G. Lima, Susanne Crocamo, Bruno R. B. Pires, Gerson M. Ferreira, Renata Binato |
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| Rok vydání: | 2021 |
| Předmět: |
animal structures
Microarray QH301-705.5 Receptor ErbB-2 Breast Neoplasms Biology Catalysis Article Inorganic Chemistry Immune system breast cancer Downregulation and upregulation Cell Line Tumor HER2 Gene expression Gene silencing Humans Physical and Theoretical Chemistry Biology (General) Molecular Biology Transcription factor QD1-999 Spectroscopy Gene knockdown Organic Chemistry Interleukin-17 Twist-Related Protein 1 IL17 Nuclear Proteins General Medicine Computer Science Applications Gene Expression Regulation Neoplastic Chemistry Cancer research gene expression Female Signal transduction Signal Transduction Twist1 |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 22 International Journal of Molecular Sciences, Vol 22, Iss 12144, p 12144 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Breast cancer (BC) is a heterogeneous disease composed of multiple subtypes with different molecular characteristics and clinical outcomes. The metastatic process in BC depends on the transcription factors (TFs) related to epithelial-mesenchymal transition (EMT), including the master regulator Twist1. However, its role beyond EMT in BC subtypes remains unclear. Our study aimed to investigate the role of Twist1, beyond EMT, in the molecular subtypes of BC. In patients, we observed the overexpression of TWIST1 in the HER2+ group. The silencing of TWIST1 in HER2+ BC cells resulted in the upregulation of 138 genes and the downregulation of 174 genes compared to control cells in a microarray assay. In silico analysis revealed correlations between Twist1 and important biological processes such as the Th17-mediated immune response, suggesting that Twist1 could be relevant for IL-17 signaling in HER2+ BC. IL-17 signaling was then examined, and it was shown that TWIST1 knockdown caused the downregulation of leading members of IL-17 signaling pathway. Taken together, our findings suggest that Twist1 plays a role on IL-17 signaling in HER2+ BC. |
| Databáze: | OpenAIRE |
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