Phytochrome-interacting factor (PIF) in rapeseed (Brassica napus L.): Genome-wide identification, evolution and expression analyses during abiotic stress, light quality and vernalization
| Autor: | Juncen Liu, Mei Guan, Weiping Wang, Chunyun Guan, Mingyao Yao, Yan Liu, Wenqian Li, Xin He |
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| Rok vydání: | 2020 |
| Předmět: |
Light
02 engineering and technology Biology Genes Plant Biochemistry Synteny 03 medical and health sciences Structural Biology Gene Expression Regulation Plant Stress Physiological Arabidopsis Brassica rapa Basic Helix-Loop-Helix Transcription Factors Promoter Regions Genetic Molecular Biology Gene Phylogeny 030304 developmental biology Plant Proteins Genetics 0303 health sciences Binding Sites Phytochrome Abiotic stress Brassica napus Promoter General Medicine Vernalization 021001 nanoscience & nanotechnology biology.organism_classification Brassica oleracea 0210 nano-technology Transcriptome Protein Binding Signal Transduction |
| Zdroj: | International journal of biological macromolecules. 180 |
| ISSN: | 1879-0003 |
| Popis: | Phytochrome-interacting factors (PIFs) are members of basic helix-loop-helix (bHLH) transcription factors and the primary partners of phytochromes (PHY) in light signaling. PIFs interact with the Pfr forms of phytochrome to play an important role in the pathways of response to light and temperature in plants. In this study, 30, 12, and 16 potential PIF genes were identified in Brassica napus, Brassica rapa, Brassica oleracea, respectively, which could be divided into three subgroups. The Br/Bo/BnaPIF genes are intron-rich and similar to the PIF genes in Arabidopsis. However, unlike the AtPIFs that exist in multiple alternative-splicing forms, the majority of Br/Bo/BnaPIF genes have no alternative-splicing forms. A total of 52 Br/Bo/BnaPIF proteins have both the conserved active PHYB binding (APB) and bHLH domains. The Ka/Ks ratio revealed that most BnaPIFs underwent purifying selection. A promoter analysis found that light-related, abscisic acid-related and MYB-binding sites were the most abundant in the promoters of BnaPIFs. BnaPIF genes displayed different spatiotemporal patterns of expression and were regulated by light quality, circadian rhythms, cold, heat, and vernalization. Our results are useful for understanding the biological functions of PIF proteins in rapeseed. |
| Databáze: | OpenAIRE |
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