MicroRNA-346-5p Regulates Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells by Inhibiting Transmembrane Protein 9
| Autor: | Yi Sun, Jinglong Yan, Jinlong Liu, Yu Han, Yicai Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
Genetic Markers
Article Subject Transmembrane protein 9 Bone Marrow Cells Core Binding Factor Alpha 1 Subunit Transfection General Biochemistry Genetics and Molecular Biology stomatognathic system Western blot Downregulation and upregulation Bone Marrow Osteogenesis medicine Humans Binding Sites Osteoblasts General Immunology and Microbiology medicine.diagnostic_test Chemistry Mesenchymal stem cell Membrane Proteins Cell Differentiation Mesenchymal Stem Cells Osteoblast General Medicine Cell biology RUNX2 MicroRNAs medicine.anatomical_structure Gene Expression Regulation Sp7 Transcription Factor Medicine Alkaline phosphatase Bone marrow Research Article Signal Transduction |
| Zdroj: | BioMed Research International, Vol 2020 (2020) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2020/8822232 |
| Popis: | The molecular mechanisms how bone marrow-derived mesenchymal stem cells (BMSCs) differentiate into osteoblast need to be investigated. MicroRNAs (miRNAs) contribute to the osteogenic differentiation of BMSCs. However, the effect of miR-346-5p on osteogenic differentiation of BMSCs is not clear. This study is aimed at elucidating the underlying mechanism by which miR-346-5p regulates osteogenic differentiation of human BMSCs. Results of alkaline phosphatase (ALP) and Alizarin Red S (ARS) staining indicated that upregulation of miR-346-5p suppressed osteogenic differentiation of BMSCs, whereas downregulation of miR-346-5p enhanced this process. The protein levels of the osteoblastic markers Osterix and Runt-related transcription factor 2 (Runx2) were decreased in cells treated with miR-346-5p mimic at day 7 and day 14 after being differentiated. By contrast, downregulation of miR-346-5p elevated the protein levels of Osterix and Runx2. Moreover, a dual-luciferase reporter assay revealed that Transmembrane Protein 9 (TMEM9) was a target of miR-346-5p. In addition, the Western Blot results demonstrated that the TMEM9 protein level was significantly reduced by the miR-346-5p mimic whereas downregulation of miR-346-5p improved the protein level of TMEM9. These results together demonstrated that miR-346-5p served a key role in BMSC osteogenic differentiation of through targeting TMEM9, which may provide a novel target for clinical treatments of bone injury. |
| Databáze: | OpenAIRE |
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