Isolation and quantitative analysis of physalin D in the fruit and calyx of Physalis alkekengi L
| Autor: | Judit Hohmann, István Zupkó, Eşianu Sigrid, Eszter Laczkó-Zöld, Peter Forgo |
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| Rok vydání: | 2017 |
| Předmět: |
Physalis
Antineoplastic Agents Apoptosis Flowers 01 natural sciences High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology Calyx Botany Humans Secosteroids Spectral analysis Physalis alkekengi L General Environmental Science biology Traditional medicine Dose-Response Relationship Drug 010405 organic chemistry Plant Extracts Neoplasms Experimental biology.organism_classification 0104 chemical sciences Physalin D 010404 medicinal & biomolecular chemistry Neurology Fruit MCF-7 Cells Quantitative analysis (chemistry) Solanaceae HeLa Cells |
| Zdroj: | Acta biologica Hungarica. 68(3) |
| ISSN: | 0236-5383 |
| Popis: | Physalin D was isolated from the methanol extract of Physalis alkekengi L. fruits by combination of different chromatographic methods (CPC, TLC, HPLC). The structure was elucidated based on 1H and 13C NMR spectral analysis with the aid of 2D-correlation spectroscopy (1H, 1H-COSY, HSQC and HMBC) and comparison with literature data. The quantity of physalin D in mature and immature fruits and calyces was determined by RP-HPLC-UV method. Among the studied samples, immature calyx showed the highest content of physalin D (0.7880 ± 0.0612%), while mature calyx contained 4 times less amount (0.2028 ± 0.016%). The physalin D content of the fruit was much lower; immature fruits contained 0.0992 ± 0.0083% physalin D and mature fruits 0.0259 ± 0.0021%. The antiproliferative activity of the CHCl3 extract and its fractions was tested on three cancer cell lines (HeLa, MCF-7 and A431). The antiproliferative activity of physalin D is discussed with regard the published data. |
| Databáze: | OpenAIRE |
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